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Device for continuous measurement of breathing delta13C of soil microorganisms

A soil microorganism and measuring device technology, which is applied in the field of δ13C measuring devices, can solve the problems of low instrument observation accuracy, time-consuming and laborious, low measuring sample collection efficiency, etc., and achieves the effects of reducing labor intensity, quick and convenient use, and ensuring test accuracy.

Inactive Publication Date: 2017-01-25
INST OF GEOGRAPHICAL SCI & NATURAL RESOURCE RES CAS
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The measurement sample collection efficiency of this self-made equipment is low, and the instrument observation accuracy is also low; 13 C measurement is time-consuming and laborious, and it is difficult to achieve continuous observation

Method used

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  • Device for continuous measurement of breathing delta13C of soil microorganisms
  • Device for continuous measurement of breathing delta13C of soil microorganisms
  • Device for continuous measurement of breathing delta13C of soil microorganisms

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Embodiment 1

[0032] The present embodiment provides a soil microbial respiration δ 13 C continuous measurement device, which includes a temperature control system, a sampling system, an analysis system, a calibration system and a control system.

[0033] The temperature control system is used to control the temperature of the culture bottle; the temperature control system can realize automatic temperature control of 0-30°C, which can be realized by mature equipment on the market, for example, the temperature control system includes a constant temperature water tank and a low temperature constant temperature circulator, the constant temperature water tank is provided with a water inlet and a water outlet, the water outlet of the low temperature constant temperature circulator is connected with the water inlet of the constant temperature water tank, the water inlet of the low temperature constant temperature circulator is connected with the The water outlet of the constant temperature water ...

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Abstract

The invention discloses a device for continuous measurement of breathing delta13C of soil microorganisms. The device comprises a sampling system, an analytic system and a control system, wherein the sampling system is used for extracting a gas in a culture bottle and conveying the gas to the analytic system; the analytic system uses a calibration gas to perform calibration so as to obtain contents of CO2 and delta13C; and the control system is used for receiving data obtained by the analytic system, and controlling the sampling system. According to the device disclosed by the invention, by establishing an in-situ cultivation system with 16 incubators, continuous measurement and system integration can be achieved, functions of automatic sample injection and analysis are formed, and isotope ratios of dalta13C-CO2 and CO2 concentrations in the incubators can be measured continuously (0.3Hz); moreover, the device disclosed by the invention has a programmable temperature control function and a function of meeting simulation of a complex situation, and can use field experience for reference to establish an online calibration system and ensure the test accuracy, so that commercial mass production and full automatic operation can be achieved, the labor intensity can be reduced, and the device is quick and convenient to use.

Description

technical field [0001] The present invention relates to a delta 13 C measuring device, especially a kind of soil microbial respiration δ 13 C continuous measuring device. Background technique [0002] Both autotrophic and heterotrophic respiration components of soil respiration are regulated by substrate availability: the supply of carbohydrates transported through the phloem controls root and mycorrhizal fungal respiration, and dead organic matter controls microbial respiration. [0003] Soil microbial respiration (CO 2 )δ 13 The measurement system of C can be divided into field and indoor measurement system. At present, there is no commercial supporting equipment, and the existing equipment is self-made by researchers. [0004] The measurement sample collection efficiency of this self-made equipment is low, and the instrument observation accuracy is also low; 13 C measurement is time-consuming and laborious, and it is difficult to achieve continuous observation. Co...

Claims

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Application Information

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IPC IPC(8): G01N21/3518G05D23/20G05B19/418G01N1/24G01N1/34
CPCY02P90/02G01N21/3518G01N1/24G01N1/34G05B19/418G05D23/20
Inventor 温学发
Owner INST OF GEOGRAPHICAL SCI & NATURAL RESOURCE RES CAS
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