Preparation method of superior microorganism solid inoculants used for river water pollution management
A solid inoculum and microorganism technology, applied in the method of using microorganisms, water/sludge/sewage treatment, polluted waterway/lake/pond/river treatment, etc., can solve the problem of unfavorable internal microorganisms to obtain nutrients and dissolved oxygen. , affecting the effect and efficiency of water pollution control, the lack of toughness of chitosan, etc., to achieve the effect of facilitating the transfer and exchange of substances, improving the concentration and stability of microorganisms, and reducing the breakage rate
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[0020] The present invention will be further described below in conjunction with embodiment.
[0021] The method for preparing the solid bacterial agent of advantageous microorganisms for river water pollution control comprises the following steps:
[0022] (1) Domestication of microorganisms: collect the river water samples and sediments to be treated, mix them evenly according to the ratio of sediment (g): water sample (ml) = 1:2, and shake them in the Erlenmeyer flask for 10 minutes to obtain the domestication stock solution , put the domestication stock solution aside for later use; divide 100ml enrichment medium into six 250ml Erlenmeyer flasks; add 10ml of the supernatant of the acclimatization stock solution to the first Erlenmeyer flask, and carry out aerated culture at 30°C. For 5 days, use a sterile pipette to draw 10ml of the solution in the Erlenmeyer flask to another Erlenmeyer flask for cultivation, and after 5 consecutive transfers, obtain a microbial mixed cult...
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