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A drug screening biochip with air chambers

A biochip and gas chamber technology, applied in drug screening, compound screening, bioreactors/fermenters for specific purposes, etc., can solve the problems of not being able to observe changes in cell morphology in real time, and achieve the effect of ensuring reliability

Active Publication Date: 2018-06-26
河南多沃生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, traditional drug screening chips cannot observe the morphological changes of cells under the action of drugs in real time.

Method used

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  • A drug screening biochip with air chambers
  • A drug screening biochip with air chambers
  • A drug screening biochip with air chambers

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment 1

[0018] refer to figure 1 The chip shape structure, the fluid channel of the lower chip 20 has a height of 800 μm and a width of 300 μm. Each culture area 7 has a diameter of 5 mm, and a "U"-shaped structure 16 with a width of 60 μm semicircular arc wall in the middle, which is 5 μm away from the inner wall of the cylindrical culture area 7 .

[0019] The operation steps are as follows: First, use a syringe pump to feed high-purity inert gas into the gas inlet 3, the gas reaches the gas chamber 9 through the gas chamber channel 4, and then discharges the impurity gas through the gas outlet 14. Then use a syringe pump to feed three-stage deionized water into the two "V"-shaped inlets 1 and 217 of the chip, and the deionized water enters through the turbine structure 5 and the arc-shaped channel 16. The cultivation zone 7 bypasses the "U"-shaped structure 16, enters the waste liquid pool 10 through the arc channel 2 15, continues to flow into the discharge channel 11, and sucks ...

specific Embodiment 2

[0020] refer to Figure 1-Figure 3 The chip shape structure, the fluid channel of the lower chip 20 has a height of 1000 μm and a width of 600 μm. Each culture area 7 has a diameter of 2 mm, and a "U"-shaped structure 16 with a width of 30 μm and a semicircular arc wall in the middle, which is 5 μm away from the inner wall of the cylindrical culture area 7 .

[0021] The operation is the same as in the specific embodiment one.

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Abstract

The invention relates to a drug screening biological chip with an air chamber. The drug screening biological chip with the air chamber is formed by two groups of sample injection ports, two cultivating areas, a commonly used air chamber, a waste liquor pool and a bended connecting channel, and is characterized in that structures starting from an air inlet, communicating an air chamber channel and an air chamber, ending at an air outlet are processed on an upper piece of the chip, and are arrayed in a connecting straight line; two groups of V-shaped sample injection channels and the two cultivating areas are symmetrically processed on a lower piece, corresponding to two sides of the connecting straight line of the upper piece; the waste liquor pool, a discharging channel and the outlet are processed on the lower piece, corresponding to the connecting straight line of the upper piece. According to the drug screening biological chip with the air chamber provided by the invention, the biological chip is utilized, a favorable cell culture basis is achieved, a microfluidic technology and a cell culture technology are combined so as to be applied in the field of drug screening through the improvement of the structure, and the same cell growth environment is provided on one chip, so that the aim that under the condition of not changing other variables, a category or dosage of a drug is changed singly, and drugs are screened through observing the change of physical signs such as cellular morphology under a drug effect is achieved.

Description

technical field [0001] The invention relates to a novel biological microanalysis chip for drug screening. In terms of use, it is a novel drug screening biological chip with an air chamber used in a living cell culture system. technical background [0002] High-throughput drug screening refers to experimental methods based on molecular and cellular levels, using microplates as experimental tool carriers, using automated operating systems to perform experimental procedures, using sensitive and fast detection instruments to collect experimental data, and using computers to analyze The experimental data is analyzed and processed, tens of millions of samples are tested at the same time, and the technical system of the whole system is supported by the corresponding database. High-throughput drug screening system is an important field of new drug development research in innovative drug screening, and has been widely used in the screening of biological activity of candidate compound...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00
CPCB01L3/5027B01L2200/10C12N2503/02G01N33/5026G01N2500/10
Inventor 廖晓玲徐文峰罗贤解文月
Owner 河南多沃生物科技有限公司
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