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Manufacturing method for early embryo presentation model of mouse

A technology for displaying models and making methods, applied in the field of popular science display, can solve problems such as inability to systematically display and display, the number of days for display model development is not fixed, and the test microscopic

Inactive Publication Date: 2017-01-11
INST OF ZOOLOGY CHINESE ACAD OF SCI
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Problems solved by technology

However, the field of basic research in life sciences is a very complex field, and most of the experiments involved are relatively microscopic and cannot be displayed systematically
Since the process of embryonic development is in vivo, it is very difficult to observe the process of embryonic development intuitively and conveniently at present, and there is no good display method to help us observe and study the complete development process of animals in vivo
[0003] In the past, similar popular science teaching aids used mice or human (aborted) fetuses, which were fixed in formalin solution to make display models. The display models made by this method could not track and display the whole process of life development because the number of days of development was not fixed. , It is ethically controversial to use human aborted fetuses for popular science education, and it is not suitable for use as popular science exhibits and teaching aids for children or young students, and needs further improvement

Method used

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Embodiment Construction

[0015] The present invention will be described in detail below, but it should be noted that these embodiments do not limit the present invention, and the functions, methods, or structural equivalent transformations or substitutions made by those skilled in the art according to these embodiments belong to this invention. within the scope of protection of the invention.

[0016] This embodiment provides a method for making a mouse early embryo display model, comprising the following steps:

[0017] a) newborn mice from 8.5 days of gestational age were placed in 4% PFA for at least 24 hours;

[0018] b) Prepare a glass petri dish with a diameter of 20-30 cm and clean it;

[0019] c) Make 200 ml of 1% agar, heat and melt in a microwave oven and pour it into a glass dish;

[0020] d) After the agar is in a semi-solidified state, put the mouse into a glass dish. Generally, four specimens can be placed in one dish;

[0021] e) After the agar is completely solidified, cut off the s...

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Abstract

The invention belongs to the technical field of popular science display, specifically relates to a manufacturing method for early embryo presentation model of mouse. The method comprises the steps of fetching newly born mouse with 8.5 days of gestational age, putting the mouse in 4% of PFA for 24h, preparing 200ml and 1% of agar, pouring the agar in a glass dish after being heated and melted by a microwave oven, putting the mouse specimen in the agar in semi-solidified state, cutting the specimen with the agar by the blade after the agar is completely solidified, putting the specimen into a transparent glass specimen bottle, pouring 4% of PFA in the specimen bottle for long-term storage. The embryo model is prepared by living body fixing method, agar and 4% of PFA, and then the mouse embryo in different stages can be presented vividly and completely in the manner of real specimen.

Description

technical field [0001] The invention belongs to the technical field of popular science display, and in particular relates to a method for making a mouse early embryo display model. Background technique [0002] With the development of science and technology, science popularization has become more and more important. However, the field of basic life science research is a very complex field, and most of the experiments involved are relatively microscopic and cannot be displayed systematically. Since the process of embryonic development is in vivo, it is very difficult to observe the process of embryonic development intuitively and conveniently at present, and there is no good display method to help us observe and study the complete development process of animals in vivo. [0003] In the past, similar popular science teaching aids used mice or human (aborted) fetuses, which were fixed in formalin solution to make display models. The display models made by this method could not...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G09B23/36
CPCG09B23/36
Inventor 周琪李天达王柳李伟吕卓夏英杰
Owner INST OF ZOOLOGY CHINESE ACAD OF SCI
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