Plant leaf resistance distribution measurement method and application thereof

Abstract

The invention discloses a plant leaf resistance distribution measurement method which specifically comprises the following steps: two microelectrode array boards are manufactured, a plant leaf is clamped between the two microelectrode array boards, a voltage is applied on an electrode wire on one of the microelectrode array boards or a current let into the electrode wire on one of the microelectrode array boards, corresponding electric response signals are collected on an electrode wire on the other of the microelectrode array boards, and the collected electric response signals are processed; a resistance value of an point, where the electrode wires of the two microelectrode array boards intersects, of the leaf is obtained; dot matrix distribution of leaf resistance can be further obtained; the invention also discloses application of the above method. According to the plant leaf resistance distribution measurement method and the application thereof, plant leaf resistance distribution can be obtained via microelectrode arrays, tissue and cells can be prevented from damage, accurate measurement can be realized, a plant water depletion degree can be sensitively and accurately determined, and plants can be timely and precisely irrigated in a convenient manner; on-site measurement of leaf resistance distribution, water depletion conditions and a function state can be realized; the method is simple and convenient and is easy to popularize.

Description

technical field [0001] The invention belongs to the technical field of agricultural applications, and relates to a method for measuring the resistance distribution of plant blades, and also relates to the application of the above method. Background technique [0002] Plant leaf tissue is composed of various cells of different shapes and a large number, which can be divided into the apoplast composed of the cell wall and interstitium of each cell and the coplasma composed of the protoplasm of adjacent cells run through by plasmodesmata body. Both the apoplast and the symplast have resistance, and their resistance and its changes are related to the functional state of the cell and the regulation of cell metabolism. Measuring and analyzing the resistance information of plant leaves is very important for the study of the functional state of plants, stress resistance evaluation, environmental monitoring and intelligence. Many areas such as new agricultural technologies are of gr...

AI Technical Summary

Problems solved by technology

However, it is difficult to measure it in vivo; the change of plant stem sap flow and stem xylem water column breakage during water shortage and the change of stem diameter are widely studied as the information of plant water demand

However, these methods are complicated to measure and have large errors, so they are not suitable for the observation of plant leaves.

So far, no breakthrough has been made in the research of accurately obtaining plant water demand information and achieving continuous and automatic monitoring with plants as objects, and no corresponding technical products have appeared.

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