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Application of menstrual blood stem cells in preparation of drugs for treating intrauterine adhesion

A technique of intrauterine adhesion and stem cells, applied in the field of biomedicine

Pending Publication Date: 2017-01-11
SHENGJING HOSPITAL OF CHINA MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Based on the advantages of menstrual blood stem cells, it is expected to be applied to the preparation of drugs for the treatment of intrauterine adhesions, but there has been no relevant report so far

Method used

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  • Application of menstrual blood stem cells in preparation of drugs for treating intrauterine adhesion
  • Application of menstrual blood stem cells in preparation of drugs for treating intrauterine adhesion
  • Application of menstrual blood stem cells in preparation of drugs for treating intrauterine adhesion

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Embodiment 1

[0020] From a humanitarian point of view, patients are more inclined to collect autologous menstrual blood stem cells to prepare drugs to treat their own intrauterine adhesion diseases. Menstrual blood stem cells can be selected from the body for drug preparation.

[0021] 1. In vitro isolation and culture of autologous stem cells.

[0022] 1. Equipment, materials and reagents used: ultra-clean bench, CO 2 Incubator, inverted microscope, centrifuge (purchased from Eppendorf), constant temperature water bath (purchased from JULABO), B-ultrasound machine, cell culture bottle, centrifuge tube (50 mL, 15 mL), straw, 5 mL syringe, artificial insemination Catheter, embryo transfer tube, blood collection needle, DMEM-F12 culture medium (purchased from HyClone Company, product number: SH30023), penicillin, streptomycin (purchased from SIGMA Company, product number: V900929), 0.25% trypsin (purchased from SIGMA Company , product number: T4049), phosphate buffer saline (PBS, purchased...

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Abstract

The invention belongs to the field of biological medicine, and particularly relates to application of menstrual blood stem cells in preparation of drugs for treating intrauterine adhesion. A preparation method of the menstrual blood stem cells comprises the following steps: diluting an extracted menstrual blood sample by using PBS, slowly adding to the upper layer of a nuclear red blood cells membrane, centrifuging and separating a mononuclear cell layer, removing supernate, resuspending a complete culture solution for cell precipitation, inoculating in a cell culture bottle, and culturing in a culture box with 5% of CO2 at the temperature of 37 DEG C; when cells grow all over to 80-90%, carrying out digestion passage with 0.25% of trypsin, and after cytoplasm retracts and gaps among cells are increased, adding the complete culture solution to terminate digestion; repeatedly blowing and beating a bottle wall to form cell suspension; sucking the cell suspension into a centrifuge tube, centrifuging and removing supernate; adjusting cell concentration by the complete culture solution for cell precipitation, and inoculating in a culture bottle; and culturing in a culture box with 5% of CO2 at the temperature of 37 DEG C, wherein culture passage numbers are 2-3. The cells are high in survival rate, stable in character, easy to obtain and easy to amplify, and has remarkable treatment effect on intrauterine adhesion.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to the application of menstrual blood stem cells in the preparation of medicines for treating intrauterine adhesions. Background technique [0002] The endometrium plays an important role in maintaining female physiological characteristics and fertility. It has a high regenerative capacity and proliferative activity. Any factor that causes endometrial destruction can cause intrauterine adhesions. With the popularization of frequent uterine operations and hysteroscopic surgery, the incidence and detection rate of intrauterine adhesions (IUA) caused by endometrial injury are increasing year by year, and it has become the second most common cause of secondary infertility in women. Two reasons. IUA is due to the formation of fibrous scars after the endometrial basal layer is damaged, and repair obstacles lead to the partial or complete adhesion of the uterine cavity. Asherman sy...

Claims

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Application Information

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IPC IPC(8): A61K35/14C12N5/078A61P15/00
CPCA61K35/14C12N5/0634C12N2509/00
Inventor 谭季春李萍萍赵冬妮李小妮李雅璇
Owner SHENGJING HOSPITAL OF CHINA MEDICAL UNIV
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