Mycoplasma urealytium immunogold labeling detection kit and detection method

Abstract

The invention provides a mycoplasma urealytium immunogold labeling detection kit and a detection method. The kit comprises a sample pad, a colloidal gold pad, a nitrocellulose membrane, water absorption filter paper and a reaction supporting substance; the sample pad is arranged at the bottom end of the reaction supporting substance, the top end of the sample pad presses the bottom end of the colloidal gold pad and is stuck to the bottom end of the colloidal gold pad, the top end of the colloidal gold pad presses the bottom end of the nitrocellulose membrane and is stuck to the bottom of the nitrocellulose membrane, and the top end of the nitrocellulose membrane is pressed by the bottom end of the water absorption filter paper and stuck to the bottom end of the water absorption filter paper. The detection processes of mycoplasma urealytium in a human urinary or genital tract secretion sample can be simplified. By utilizing the detection kit to detect the mycoplasma urealytium, detection operation has no dependency on any experiment instrument or environment or operating personnel, operation is easy and convenient, the detection cycle is short, and interpretation is easy; in addition, special instruments or devices are not needed, professional training is not needed, the adaptability is high, and monitoring and inspecting are conveniently conducted at anytime anywhere.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a gold standard detection kit and detection method for ureaplasma urealyticum. Background technique [0002] Ureaplasma urealyticum (UU) is a prokaryotic microorganism whose volume is between that of bacteria and viruses. It belongs to Mycoplasma Molliculata and lacks cell wall and lipopolysaccharide. It is often stained lavender by Dienes staining. Its molecular weight is small, about 750-947kbp, which is only one-fifth of Escherichia coli, and its synthesis and metabolism are limited. It is the smallest prokaryotic cell known to be able to grow and reproduce on an inanimate medium. In 1954, Shepard et al. first isolated Ureaplasma urealyticum (UU) from the urethral secretions of male patients with nongonoccal urethritis (NGU). [0003] Human Ureaplasma urealyticum contains at least 14 serotypes, which can be determined according to genome size, 16SrRNA, 16S-23SrRNA intergen...

AI Technical Summary

Problems solved by technology

However, these detection methods all need to rely on corresponding instruments and equipment, and require the use of liquid reagents, etc., which are not convenient for on-site detection, and the storage temperature and use of reagents are also limited, and it is not convenient to obtain experimental results quickly and easily.

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