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Taq Plus polymerase kit and usage method thereof

A kit and polymerase technology, applied in biochemical equipment and methods, enzymology/microbiology devices, bioreactor/fermenter combinations, etc., can solve the problem of low reaction sensitivity, low amplification efficiency, limited PfuDNA polymerase Use and promotion, etc., to achieve the effect of fast amplification, simple operation, and shortening the time for selecting reagents

Inactive Publication Date: 2017-01-04
周辉
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, high-fidelity polymerase has relatively high requirements on the structure, purity and concentration of primers and samples, and the reaction sensitivity is low, and the amplification efficiency is low, which limits the use and promotion of Pfu DNA polymerase

Method used

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  • Taq Plus polymerase kit and usage method thereof
  • Taq Plus polymerase kit and usage method thereof
  • Taq Plus polymerase kit and usage method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment example

[0047] Implementation case: Amplification of various large fragments and complex templates.

[0048] 1. PCR system

[0049]

[0050]

[0051] *Optional

[0052] 2. PCR conditions

[0053]

[0054] X*: predicted product length in kb.

[0055] 3. 1% agarose gel electrophoresis: detection of bands.

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Abstract

The invention relates to a Taq Plus polymerase kit and a usage method thereof. The Taq Plus polymerase kit comprises a square box, a foam pad and reagent bottles, wherein the foam pad is arranged in the square box and is provided with four holes, the reagent bottles include a reagent bottle 1, a reagent bottle 2, a reagent bottle 3 and a reagent bottle 4 and are put in the four holes, the reagent bottle 1 contains Taq Plus polymerase, the reagent bottle 2 and the reagent bottle 3 contain a buffer solution, and the reagent bottle 4 contains an enhancer solution. The kit is simple in operation, pollution produced in the operating process is reduced, meanwhile the reagent bottles used in the kit are transparent, bottle caps are different in color and are provided with round marks, the time for selecting reagents is shortened, and the Taq Plus polymerase kit is convenient to use.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a Taq Plus polymerase kit and a use method thereof. Background technique [0002] Polymerase Chain Reaction (PCR) is a method for synthesizing double-stranded DNA in vitro. The basic principle of the technology is based on the mechanism of cell replication established by the DNA double helix model and the characteristics of double-stranded DNA replication in vitro. of. Using DNA as a template and small fragments of DNA as primers, dNTPs are synthesized into new strands of DNA under the catalysis of DNA polymerase. Therefore, DNA polymerase is the key to realize DNA replication. Therefore, Taq DNA polymerase is widely used in PCR technology, but Taq DNA polymerase lacks the correction function. In the extension reaction of PCR chain, it is often terminated due to mismatch or high GC content of DNA template, and cannot recognize and excise unpaired DNA. The nucleotides at the end of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12N15/10
CPCC12Q1/686C12P19/34C12Q2521/101
Inventor 周辉
Owner 周辉
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