Uses of oleanolic acid in induction of differentiation of human embryonic stem cells into germ-like cells

A technology of human embryonic stem cells and oleanolic acid, applied in the field of oleanolic acid inducing the differentiation of human embryonic stem cells into germ-like cells

Inactive Publication Date: 2016-12-21
CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Abstract
  • Description
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Problems solved by technology

[0006] However, whether OA has the possibility of inducing hESCs to differentiate to germ cells in vitro has not been reported yet.

Method used

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  • Uses of oleanolic acid in induction of differentiation of human embryonic stem cells into germ-like cells
  • Uses of oleanolic acid in induction of differentiation of human embryonic stem cells into germ-like cells
  • Uses of oleanolic acid in induction of differentiation of human embryonic stem cells into germ-like cells

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Experimental program
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Effect test

experiment example 1

[0027] Experimental example 1 Inducing solution of the present invention induces umbilical cord mesenchymal stem cells to differentiate into nerve cells

[0028] 1 material

[0029] 1.1 Cells: human embryonic stem cells h9 were purchased from the Shanghai Stem Cell Bank of the Chinese Academy of Sciences, the number of the Chinese Academy of Sciences Stem Cell Bank: SCSP-302, the feeder layer cells NIH3T3: ATCC number: ATCCCRL-1658

[0030] 1.2 Drugs and reagents: Oleanolic acid (OA): prepared by School of Pharmacy, Chengdu University of Traditional Chinese Medicine (purity 98%), basic fibroblast growth factor (bFGF) was purchased from R&D, β-mercaptoethanol was purchased from Invitrogen, DMEM / F12, fetal bovine serum (FBS), serum substitutes, and non-essential amino acids were purchased from Gibco. Primary antibody: anti-Scp3, secondary antibody: goat anti-rabbit, purchased from Santa Cruz. Hoechst 33258: Beyond, cDNA synthesis kit (Fermentas, K1632), qPCR kit (Roche, 04913...

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Abstract

The present invention discloses uses of oleanolic acid in induction of differentiation of human embryonic stem cells into germ-like cells. The invention further discloses an induction liquid and an induction differentiation method for inducing the differentiation of human embryonic stem cells into germ-like cells. According to the present invention, with the oleanolic acid, the differentiation of the human embryonic stem cells into the germ cells can be promoted; and the prepared germ-like cells have the potential in differentiation into the germ cells, can be prepared into the raw material for the in vitro fertilization technology, provide the new thinking for the treatment of infertility diseases, and have important clinical application value.

Description

technical field [0001] The invention relates to the use of oleanolic acid in inducing the differentiation of human embryonic stem cells into germ-like cells. Background technique [0002] In recent years, due to serious environmental pollution, increased work pressure, changes in eating habits, reproductive system diseases and other factors that adversely affect normal conception, the number of infertile patients is on the rise, with an incidence rate of about 12.5% ​​to 15%. increasing trend year by year. Therefore, the World Health Organization (WHO) announced that infertility, cardiovascular disease, and tumors are listed as the three major diseases that affect human life and health today. Since the world's first test-tube baby was born in 1978, the introduction of assisted reproductive technology (ART) represented by in vitro fertilization-embryo transfer (IVF-ET) has provided greater possibilities for the successful treatment of infertility. Research on assisted pregn...

Claims

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Application Information

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IPC IPC(8): C12N5/075
Inventor 陆华高小平刘志斌周天秀米玲刘芊辰
Owner CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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