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Preparation method of bacterial agent for purifying pesticide residues in fruits and vegetables

A technology of pesticide residues and bacterial agents, applied in chemical instruments and methods, bacteria, detergent compositions, etc., can solve the problems of high price, late start, not recognized by domestic authoritative organizations, etc., and achieve low production cost and residual effect Good results

Active Publication Date: 2020-01-14
山东知柚信息科技有限公司
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  • Claims
  • Application Information

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Problems solved by technology

At present, there are some products of fruit and vegetable pesticide residue purification bacteria agents on the market, but the production and market are mainly controlled by large foreign biological companies, the price is high, and most of them have not been recognized by domestic authorities
Domestic purification bacteria agents started relatively late, restricted by production costs, culture conditions, detection methods and consumer awareness, they have not yet been popularized

Method used

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  • Preparation method of bacterial agent for purifying pesticide residues in fruits and vegetables
  • Preparation method of bacterial agent for purifying pesticide residues in fruits and vegetables

Examples

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Embodiment 1

[0031] Embodiment 1, a kind of preparation method of fruit and vegetable pesticide residue purifying bacterial agent, comprises the following steps:

[0032] a. Select the lactic acid bacteria strain: take the strain 10574 stored in the refrigerator at 4°C, put it on the ultra-clean table, and let it stand for 2 hours. : CGMCC No.10574;

[0033] b. Bacterial culture medium (seed medium): 7.5g of yeast extract, 7.5g of peptone, 10g of glucose, 2g of potassium dihydrogen phosphate, 0.5mL of Tween 80, set the volume to 1000mL, divide into 500mL conical flasks with 100mL, 121 ℃ autoclave for 35 minutes;

[0034] c. Fermentation medium: 10g of yeast extract, 10g of peptone, 40g of corn steep liquor, 10g of sucrose, 5g of calcium carbonate powder, dilute to 1000mL with water, stir evenly, adjust the pH to 6.5, put into a 3000mL triangular flask, and autoclave at 121°C After 35 minutes, store in the refrigerator at 4°C;

[0035] After testing, it was found that the above medium ha...

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Abstract

The invention discloses a preparation method of a fruit / vegetable pesticide residue purification microbial inoculant. The preparation method comprises the following steps: after culturing a lactobacillus strain, carrying out centrifugal cleaning, and adding a sodium chloride water solution, wherein the lactobacillus strain is collected by China General Microbiological Culture Collection Center (CGMCC for short), and the collection number is CGMCC No.10574. The sodium chloride solution added into the lactobacillus strain can reduce the damage of low temperature to the strain, and can also inhibit growth of the lactobacillus. The strain is easy to control and convenient and easy to acquire. The microbial inoculant has the advantages of low production cost, high safety, no harm and favorable pesticide residue purification effect.

Description

[0001] field of invention [0002] The invention relates to a bacterial agent for purifying fruit and vegetable pesticides, in particular to a method for preparing the bacterial agent for purifying fruit and vegetable pesticides, and belongs to the field of biotechnology. Background technique [0003] Food safety is one of the most important concerns of consumers. my country has implemented a new food law since 2015, which is particularly strict on food safety issues. However, in order to ensure the yield of fruits and vegetables, the use of pesticides in planting cannot be avoided. For example, the incident of thiamethoxam residues in onions in 2014 attracted great attention from the General Administration of Quality Supervision, Inspection and Quarantine, and it is still a monitoring item for onions exported to Japan. Fruit and vegetable products are essential food for consumers in daily life. Some of them are even eaten directly after washing. If there are pesticide residue...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C11D3/38
CPCC11D3/381C12N1/20
Inventor 丁葵英郭礼强王振刚孙军许文娟刘永强
Owner 山东知柚信息科技有限公司
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