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Method for preparing polyglutamic acid by means of combination of fermentation waste thalli and agricultural wastes

A technology for agricultural waste and polyglutamic acid, applied in the directions of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problem of high cost of fermentation medium, reduce costs, improve leaching rate, and improve overall production The effect of efficiency

Active Publication Date: 2016-12-07
内蒙古阜丰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the defect of high cost of fermentation medium in the prior art, and provide a method for jointly preparing polyglutamic acid by using fermentation waste cells and agricultural waste

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The method for jointly preparing polyglutamic acid by using waste fermentation cells and agricultural waste includes the following steps:

[0027] Step 1) Hydrolyze and ferment waste cells: use microbial fermentation to prepare glutamic acid fermentation broth, filter and collect the fermentation waste cells, the filtrate is used to extract glutamic acid, and the mother liquor after glutamic acid extraction is used for future use;

[0028] Dry the waste fermentation cells, pulverize them into powder, and place them in a reaction tank, add 5mol / L hydrochloric acid, subject to the raw materials, stir and hydrolyze at 60℃ for 24 hours, stirring at 300 Rpm / min, after the reaction is terminated, neutralize the residual hydrochloric acid with ammonia water, control the pH of the solution to 7.0, and obtain the hydrolysate;

[0029] Step 2) Concentrate the mother liquor: Concentrate the mother liquor obtained in step 1) to a concentrate with a glutamic acid content of 12g / L;

[0030] ...

Embodiment 2

[0039] The fermentation medium used in Example 2 was: glucose 30g / L, corn steep liquor 20g / L, yeast extract 10g / L, sodium glutamate 25g / L, ammonium sulfate 6g / L, potassium dihydrogen phosphate 0.2g / L, Water magnesium sulfate 0.1g / L, ferrous sulfate 0.1mg / L; other processes are the same as in Example 1.

Embodiment 3

[0041] The yield of polyglutamic acid in the fermentation broth of Example 1-2 of the present invention (detected by ultraviolet spectrophotometry), the specific results are shown in Table 1:

[0042] Table 1

[0043] Group

[0044] Conclusion: The acid production of Example 1 of the present invention and Example 2 is not much different, and the amount of acid produced in Example 1 of the present invention is slightly higher; the cost of the fermentation medium in Example 1 of the present invention is only as much as the cost of the medium in Example 2. About 40%, and realized the transformation of waste into treasure, saving enterprise investment and increasing enterprise net income.

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PUM

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Abstract

The invention belongs to the field of amino acid production and discloses a method for preparing polyglutamic acid by means of combination of fermentation waste thalli and agricultural wastes. The method comprises the following steps of 1, hydrolyzing fermentation waste thalli; 2, concentrating a mother solution; 3, taking fermentation culture medium raw materials; 4, preparing a fermentation culture medium; 5, fermenting and preparing polyglutamic acid. Waste thalli and agricultural waste are adopted for fermentation, waste materials are turned into wealth, and enterprise investment is saved.

Description

Technical field [0001] The invention belongs to the field of amino acid production, and specifically relates to a method for jointly preparing polyglutamic acid by using waste fermentation bacteria and agricultural waste. Background technique [0002] Polyglutamic acid (abbreviated as γ-PGA or PGA) is a homogenous polypeptide formed by polymerization of glutamic acid monomers with an amide bond at the γ-position. It is water-soluble, biodegradable, non-toxic, and can be widely used in the food industry, cosmetics, health care, water treatment, wastewater treatment, sanitary products, medical and other fields. For example, it can be used as a thickener, cryoprotectant, and retarder. Release agents, drug carriers, biological adhesives, humectants, biodegradable fibers, super absorbent resins, biological flocculants and heavy metal ion absorbents. [0003] The prior art research on the fermentation process of γ-polyglutamic acid mainly focuses on the improvement of strains, and the i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/02C12R1/125
CPCC12P21/02
Inventor 卢松刘元涛许传娟聂建伟王皓王文强徐亚娟
Owner 内蒙古阜丰生物科技有限公司
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