Method for feeding hirudo nipponia adults and sustainably extracting hirudin and special equipment
A Japanese medical leech and hirudin technology, applied in chemical instruments and methods, from leech inhibitors, protease inhibitors, etc., can solve a large number of problems, achieve the effects of increasing single output, significant economic benefits, and saving resources
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Embodiment 1
[0022] Structure and usage of embodiment 1 special equipment
[0023] The special equipment for the sustainable extraction of hirudin from Japanese medical leech adults is composed of an outer cylinder 1, an inner cylinder 2 and an ultraviolet light source 3; wherein, the outer cylinder 1 is a box-shaped or barrel-shaped structure with an upper opening, and the outer cylinder 1 A support frame 1-1 is provided above, and a valve 1-2 for draining water is provided at the bottom of the outer cylinder 1; the inner cylinder 2 is arranged inside the outer cylinder 1, and the inner cylinder 2 is a box-shaped structure or a barrel-shaped structure with an upper opening, The bottom of the inner cylinder 2 is composed of a filter bottom plate 2-1, the filter bottom plate 2-1 is a sieve plate or screen mesh with an aperture of 20-100 mesh, the inner cylinder 2 is connected with a hook 2-2; the outer light source 3 is arranged on the outer cylinder 1 and the inner The top of the opening a...
Embodiment 2
[0032] The influence of embodiment 2 different emetic agents on Japanese medicine hirudin extraction amount
[0033] Weigh 30kg of adult Japanese leeches, and divide them into 3 groups, respectively named group A, group B and group C, 10kg in each group; first fast the fed adult leeches at 22-25°C for 15 Day, then adopt special equipment under 25-29 ℃ environmental conditions, refer to the method described in Example 1 to extract hirudin. Group A was fed with an emetic with a weight ratio of zinc sulfate, acetic acid and water of 2:5:100; Group A was fed with an emetic with a weight ratio of zinc sulfate, 95% alcohol and water with a weight ratio of 2:5:100; Group C was fed with An emetic with a weight ratio of zinc sulfate and water of 2:100. After each group collects the hirudin extract, add starch and chitosan in a weight ratio of 10:2 to form a stabilizer, and the addition amount is 100 g for each group. Immediately after spray drying, the crude extract of hirudin was ob...
Embodiment 3
[0034] The influence of embodiment 3 different inducing liquid Japanese medicine Hirudin extract amount
[0035] (1) Preparation of induction solution
[0036] Weigh 1000g of fresh eel, cut into pieces and pulverize, put into 5000ml of water, filter through 100 mesh, collect the water, and add 200g of glucose to the water to prepare the eel induction solution;
[0037] Weigh 1000 g of animal liver, cut into pieces and pulverize, put into 5000 ml of water, filter through 100 mesh, collect the water, and add 200 g of glucose to the water to prepare viscera induction solution;
[0038] Weigh 1000g of fresh and alive earthworms, cut into pieces and pulverize, drop into 5000ml water, filter through 100 mesh, collect the water liquid, and add 200g glucose to the water liquid to prepare the earthworm induction liquid;
[0039] (2) Investigation of induction solution
[0040] Weigh 30kg of adult Japanese leeches, and divide them into 3 groups, respectively named group I, group II an...
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