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Breeding method of puffer fish

A technology for fish fry and puffer fish, which is applied in the field of puffer fish fry breeding, can solve the problems such as the yield and survival rate need to be improved, and achieve the effect of improving hatching rate and hatching speed, easy to realize, and good technical effect

Inactive Publication Date: 2016-12-07
TIANJIN AGRICULTURE COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The conventional breeding method of puffer in the prior art all needs to be improved aspect output and survival rate

Method used

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  • Breeding method of puffer fish
  • Breeding method of puffer fish

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Experimental program
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Effect test

Embodiment 1

[0029] 1. The selection, cultivation and matching of the parents of the two sexes, and the matching attempt after the molecular markers are tried in the later stage.

[0030] 2. Technical route for fry breeding.

[0031] ① Artificial aphrodisiac In order to increase the spawning capacity of the female parent and obtain a large number of fry, artificial aphrodisiac must be carried out. The aphrodisiac drugs use chorionic gonadotropin (HCG) plus luteinizing hormone analog (LRH-A2) and dioconone (DOM), the dose is HCG1800IU+LRH-A28μg+DOM1.5mg per kg body weight, twice Chest injection. One-third of the full dose is injected with the first injection, and the remainder is injected with the second injection. The dose for male fish was half of that for female fish. Effect time, the gonads are well developed, the water temperature is 21-22°C, estrus and ovulation will occur 18-24 hours after injection. In order to grasp the best time for artificial insemination, the female fish and...

Embodiment 2

[0036] A puffer fish fry breeding method, comprising the following steps:

[0037] 1) Inject the aphrodisiac drug to the female puffer fish at a dose corresponding to 1 part of the aphrodisiac drug per kilogram of body weight of the female puffer fish. The injection is divided into 2 injections, the first injection is 25% of the total weight of the aphrodisiac drug, and the second injection is the remaining amount ;

[0038] 2) inject the aphrodisiac drug to the male puffer fish at a dose corresponding to 0.5 parts of aphrodisiac drug per kilogram of body weight of the male puffer fish. The injection is divided into 2 shots, the first injection is 25% of the total weight of the aphrodisiac drug, and the second injection is the remaining amount ;

[0039] 3) Place the injected female pufferfish and male pufferfish in different water environments, keeping the water temperature at 21°C;

[0040] 4) Take the female puffer puffer 18 hours after injection in step 1, collect eggs, ...

Embodiment 3

[0055] A puffer fish fry breeding method, comprising the following steps:

[0056] 1) Inject the aphrodisiac drug to the female puffer fish at a dose corresponding to 1 part of the aphrodisiac drug per kilogram of body weight of the female puffer fish. The injection is divided into 2 injections, the first injection is 40% of the total weight of the aphrodisiac drug, and the second injection is the remaining amount ;

[0057] 2) inject the aphrodisiac drug to the male puffer fish at a dose corresponding to 0.5 parts of aphrodisiac drug per kilogram of body weight of the male puffer. The injection is divided into 2 shots, the first injection is 40% of the total weight of the aphrodisiac drug, and the second injection is the remaining amount ;

[0058] 3) Place the injected female pufferfish and male pufferfish in different water environments, keeping the water temperature at 22°C;

[0059] 4) Take the female Pufferfish 24 hours after the injection in step 1, collect eggs, put ...

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Abstract

The invention provides a method for puffer fish fry breeding. The method starts from parental selection, and successively optimizes the way of aphrodisiac, timing of egg retrieval, fertilization conditions, hatching method and fry cultivation process. Specific aphrodisiac drugs and injection methods can help increase the ovulation volume of female fish. The timing of egg collection 18-24 hours after injection is selected because the drug effect is most significant during this time period. Pufferfish are placed separately to avoid the problem of low natural mating efficiency in the process. On this basis, the present invention matches suitable hatching conditions, defines parameters such as illumination, salinity, and dissolved oxygen, and significantly improves the hatching rate and hatching speed. The invention achieves good technical effects through innovative technical improvement, and at the same time has low cost and is easy to realize, so it has good promotion prospects.

Description

technical field [0001] The invention relates to the technical field of puffer fish breeding, in particular to a puffer fish fry breeding method. Background technique [0002] Pufferfish, commonly known as puffer fish, is a collective name for fish in the family Fuguidae of the bony fish class. In puffer fish breeding technology, fish fry breeding is very important. High-quality fish fry breeding technology is the technical premise to ensure the survival rate of offspring and increase production. It is also one of the basic conditions for expanding production scale and improving economic benefits. The natural reproduction of puffer fish is carried out under the constraints of external conditions such as water temperature, water flow, dissolved oxygen, light, water level changes, and sexual attraction and egg attachments. When the sensory organs of mature broodstock are stimulated under the combined action of these ecological conditions, the fish will generate an impulse, whi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00A61D19/02
CPCY02A40/81A01K61/00A61D19/02
Inventor 邢克智陈成勋郭永军王庆奎徐大为孙学亮石洪玥黄亚东
Owner TIANJIN AGRICULTURE COLLEGE
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