Aflatoxin online sample introduction analytical method
A technology of aflatoxin and analysis method, which is applied in the field of fully automatic online sample injection analysis, can solve the problems of large manual operation errors, poisoning operators, and polluting the environment, and achieves less solvent consumption, high recovery rate, and fast analysis speed Effect
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[0014] Embodiment 1. Using automatic mycotoxin pretreatment and online sampling platform and HPLC to detect aflatoxin in food
[0015] 1. Reagents and equipment
[0016] 1.1 Reagent preparation
[0017] 1.1.1 Sample extraction solvent: methanol / water (4 / 1, v / v).
[0018] 1.1.2 Preparation of 5mmol phosphate buffered saline solution (PBS) (pH7.2):
[0019] 1.1.2.1 Weigh 50.14gNa 2 HPO 4 .12H 2 O is dissolved in 700ml of water;
[0020] 1.1.2.2 Weigh 9.66gNaH 2 PO 4 .1H 2 O is dissolved in 350ml of water;
[0021] 1.1.2.3 Mix the above two solutions and add 42.5g NaCl to prepare a pH7.25mmol phosphate buffer solution.
[0022] 1.1.3 Preparation of 1mmol phosphate buffered saline solution (PBS) (pH7.2):
[0023] Take 200ml of 5mmol phosphate buffer solution (pH7.2) and dilute it in 800ml of water.
[0024] 1.1.4 Methanol / water: 40 / 60.
[0025] 1.1.5 Eluent: Take 10mL of solvent A, add it to 500mL distilled water, and mix well.
[0026] 1.1.6 HPLC mobile phase prepar...
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