Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Aflatoxin online sample introduction analytical method

A technology of aflatoxin and analysis method, which is applied in the field of fully automatic online sample injection analysis, can solve the problems of large manual operation errors, poisoning operators, and polluting the environment, and achieves less solvent consumption, high recovery rate, and fast analysis speed Effect

Inactive Publication Date: 2016-11-23
SHANGHAI IMPACT SCI INSTR
View PDF5 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method that overcomes the problems in the prior art that sample pretreatment is tedious, time-consuming, manual operation errors are large, and highly toxic standard samples and various toxic organic solvents are used in the pretreatment process to poison operators and pollute the environment. Fully automatic aflatoxin online sample injection analysis method

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Aflatoxin online sample introduction analytical method
  • Aflatoxin online sample introduction analytical method
  • Aflatoxin online sample introduction analytical method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Embodiment 1. Using automatic mycotoxin pretreatment and online sampling platform and HPLC to detect aflatoxin in food

[0015] 1. Reagents and equipment

[0016] 1.1 Reagent preparation

[0017] 1.1.1 Sample extraction solvent: methanol / water (4 / 1, v / v).

[0018] 1.1.2 Preparation of 5mmol phosphate buffered saline solution (PBS) (pH7.2):

[0019] 1.1.2.1 Weigh 50.14gNa 2 HPO 4 .12H 2 O is dissolved in 700ml of water;

[0020] 1.1.2.2 Weigh 9.66gNaH 2 PO 4 .1H 2 O is dissolved in 350ml of water;

[0021] 1.1.2.3 Mix the above two solutions and add 42.5g NaCl to prepare a pH7.25mmol phosphate buffer solution.

[0022] 1.1.3 Preparation of 1mmol phosphate buffered saline solution (PBS) (pH7.2):

[0023] Take 200ml of 5mmol phosphate buffer solution (pH7.2) and dilute it in 800ml of water.

[0024] 1.1.4 Methanol / water: 40 / 60.

[0025] 1.1.5 Eluent: Take 10mL of solvent A, add it to 500mL distilled water, and mix well.

[0026] 1.1.6 HPLC mobile phase prepar...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Volumeaaaaaaaaaa
Login to View More

Abstract

The invention discloses an online sampling analysis method of aflatoxin, which comprises the following steps: after the sample extract is filtered and diluted, the filtrate passes through an immunoaffinity column containing aflatoxin specific antibody, and the antibody is compatible with aflatoxin B1, B2 , G1, G2 have specific antigen-antibody binding, so that they are retained on the affinity column, other impurities flow out with the mobile phase, the antigen-antibody bond is broken by heating, and the target compound is eluted with water, and the eluent passes through the fully automatic fungal The toxin pretreatment and online sampling platform injects the sample into the high-performance liquid chromatograph online, and the aflatoxin is fully separated. The target compound is derivatized by a post-column derivatization system and can be detected by a fluorescence detector. Each aflatoxin can be obtained separately accurate quantification. This method breaks the antigen-antibody bond by heating, elutes the target compound with water, avoids the use of organic solvents, and is environmentally friendly; fully automatic operation not only improves the production efficiency and repeatability, but also avoids the operator from contacting the highly toxic aflatoxin Standard.

Description

technical field [0001] The invention relates to a method for detecting aflatoxin, in particular to a fully automatic online sample introduction analysis method. Background technique [0002] Mycotoxins are secondary metabolites secreted by toxin-producing fungi. Aflatoxin (Aflatoxin) is the most toxic mycotoxin and has been classified as a class I carcinogen by the Cancer Research Institute of the World Health Organization (WHO). The danger of aflatoxin is that it can damage the liver tissue of humans and animals, and can lead to liver cancer or even death in severe cases. Aflatoxin B1 is the most common in naturally contaminated food, and its toxicity and carcinogenicity are also the strongest. Aflatoxins are widely found in agricultural products such as rice, corn, peanuts, soybeans, and some meat products. For this reason, all countries in the world have stipulated the maximum allowable content of aflatoxin B1 in food and feed as a mandatory standard. Therefore, it is ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N30/14G01N30/24
Inventor 郭子媛亢磊赵学伟王宏沈利华蒋家奎徐玮
Owner SHANGHAI IMPACT SCI INSTR
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products