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Biomarker of prostatic cancer

A prostate cancer and marker technology, applied in the field of biomedicine, can solve problems such as affecting the effect of tumor treatment

Inactive Publication Date: 2016-11-23
北京致成生物医学科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, chemoradiotherapy drugs not only act on the tumor, but can also act on the healthy tissues around the tumor. Therefore, while killing the tumor, they also bring great side effects to the body, which ultimately affects the therapeutic effect on the tumor.

Method used

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  • Biomarker of prostatic cancer
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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1 High-throughput sequencing screening of differentially expressed genes

[0049] 1. Sampling

[0050] From October 2011 to December 2015, 27 tissue samples were obtained from the Department of Urology in Peking Union Medical College Hospital. All samples were confirmed by pathological examination, including 8 cases of paracancerous tissue samples and 19 cases of prostate cancer samples. Store in -80°C low temperature refrigerator.

[0051] 2. Total RNA extraction from tissue samples

[0052] use Reagent (invitrogen, Cat. No. 15596-018) was used to extract sample RNA, and the experimental operation was carried out according to the product manual. The specific operation was as follows:

[0053] After collecting the samples, freeze them in liquid nitrogen. After taking them out, put the tissue into a pre-cooled mortar for grinding. After the tissue sample is powdered:

[0054] ① Add Trizol and store at room temperature for 5 minutes;

[0055] ② Add 0.2 mL of...

Embodiment 2

[0064] Example 2 RT-PCR verification of gene expression in prostate cancer tissue and paracancerous tissue

[0065] 1. Materials

[0066] 34 cases of prostate cancer tissue samples and 8 cases of paracancerous tissue samples were obtained from prostate cancer samples in urological surgery of Peking Union Medical College Hospital from 2011 to 2015, and they were grouped and numbered. All samples were confirmed by pathological examination.

[0067] 2. Method

[0068] 2.1 Extract the total RNA from the tissue sample, the same as the extraction method in Example 1.

[0069] 2.2 Synthesis of cDNA by reverse transcription

[0070] use III Reverse Transcriptase (invitrogen, Cat. No. 18080-044) was used for cDNA reverse transcription, and the experimental operation was carried out according to the product manual. The specific operation was as follows:

[0071] Using a reverse transcription kit, 1 μg of total RNA was reverse-transcribed with reverse transcription buffer to synthe...

Embodiment 3

[0090] Example 3 Prostate cancer prognosis assessment kit preparation

[0091] Based on the primer set obtained in Example 2, the kit for detecting prostate cancer according to the present invention is assembled, and the kit includes specific amplification of one or more of EFCAB10, MBL1P, PPIEL, RAET1K, RPL13AP6 and / or THSD1P1 The primer set of the gene is shown in Table 1, specifically:

[0092] 1. The kit includes specific amplification of EFCAB10;

[0093] 2. The kit includes specific amplification of EFCAB10, MBL1P, PPIEL, RAET1K, RPL13AP6 and THSD1P1.

[0094] The primer pair for specifically amplifying the housekeeping gene (GAPDH) is shown in SEQ ID NO: 13 and SEQ ID NO: 14; it also includes a SYBR Green polymerase chain reaction system, such as PCR buffer, SYBR Green fluorescent dye, and dNTPs. The composition of described PCR buffer is 25mM KCl, 2.5mM MgCl 2 , 200mM (NH 4 ) 2 SO 4 ; Also includes normal prostate tissue cDNA: used as a negative control and detec...

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Abstract

The invention discloses a marker for detecting prostatic cancer. The marker is selected from one or more genes of EFCAB10, MBL1P, PPIEL, RAET1K, RPL13AP6 and / or THSD1P1. The invention further discloses application of the marker to preparing prostatic cancer diagnosis and prognosis products. The invention further discloses a prostatic cancer diagnosis or prognosis evaluation kit. The kit comprises one or more groups of specifically amplified primer sequences in EFCAB10, MBL1P, PPIEL, RAET1K, RPL13AP6 and / or THSD1P1. According to the marker, one or more genes are used as markers for joint detection of the prostatic cancer, so that early detection and prognosis evaluation can be quickly and effectively realized, the precision is greatly increased, and additionally, a therapeutic target and an important basis are provided for clinical applications such as gene therapy and drug therapy.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to the product application of diagnosing cancer and monitoring the therapeutic effect by the expression level of prostate cancer-related genes. Background technique [0002] Prostate cancer is one of the common male reproductive system malignancies. Worldwide, the incidence of prostate cancer ranks second among all malignant tumors in men. In the United States, the incidence of prostate cancer has surpassed that of lung cancer, becoming the first tumor that endangers men's health. The incidence of prostate cancer in Asia is far lower than that in European and American countries, but it has shown an upward trend in recent years, and the growth rate is faster than that in developed countries in Europe and America. Prostate cancer patients are mainly elderly men, usually after the age of 50, 95% occur in elderly men over 60 years old, and the incidence rate continues to increase with age. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/113
CPCC12Q1/6886C12Q2600/118C12Q2600/158C12Q2600/178
Inventor 刘昊
Owner 北京致成生物医学科技有限公司
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