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A molecular marker hf1-indel related to watermelon pulp firmness and its application

A technology for watermelon and pulp, applied in the field of molecular marker Hf1-Indel, which can solve the problem of not being able to accurately locate the hardness gene

Active Publication Date: 2019-11-12
京研益农(北京)种业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few related studies on watermelon fruit firmness genes at home and abroad, and the precise positioning of firmness genes has not yet been possible.

Method used

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  • A molecular marker hf1-indel related to watermelon pulp firmness and its application
  • A molecular marker hf1-indel related to watermelon pulp firmness and its application
  • A molecular marker hf1-indel related to watermelon pulp firmness and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Example 1. Acquisition of Molecular Marker Hf1-Indel Related to Watermelon Pulp Firmness

[0056] 1. Test materials

[0057] The test materials include paternal parent, female parent, F1 generation, RILs groups;

[0058] The male parent is: PI296341-FR, which is a typical wild type watermelon material, with high fruit hardness and low sugar content;

[0059] The female parent is: 97103, which is a typical East Asian type of watermelon cultivar, with crispy and easy-to-crack flesh and high sugar content;

[0060] The F1 generation is: the F1 generation obtained by crossing the above-mentioned PI296341-FR male parent and 97103 as the female parent;

[0061]The RILs population is: the RILs population obtained by the selfing of the above-mentioned F1 generation for multiple generations, a total of 103 strains (see Table 1 for details);

[0062] The 21 resequenced populations are: the tested watermelon core germplasm resources (see Table 2 for details);

[0063] All the ...

Embodiment 2

[0102] Embodiment 2, verification of Hf1-Indel marker

[0103] In order to further verify the linkage relationship between the Hf1-Indel marker in Example 1 and the watermelon pulp firmness gene Hf1, natural populations were used for verification. Specific steps are as follows:

[0104] 1. Test materials

[0105] The test material is a natural resource group; the natural group is: a natural group composed of 123 representative watermelon germplasm resources in 1484 watermelon resource banks (see Table 3 for details); each test material in Table 3 is Beijing Germplasm resource materials preserved in the Germplasm Resource Bank of the Vegetable Research Center of the Municipal Academy of Agriculture and Forestry Sciences.

[0106] Table 3. Individual plant materials of natural populations composed of 123 watermelon germplasm resources and the identification results of individual plant pulp hardness

[0107] (A is non-hard meat belt type in mark detection, B is hard meat belt ty...

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Abstract

The invention discloses a molecular marker Hf1-Indel related to hardness of watermelon flesh and application of the molecular marker Hf1-Indel. On the basis of a 97103 whole genome sequence and PI296341-FR resequencing information, the molecular marker Hf1-Indel related to hardness of the watermelon flesh is a closely interlocked molecular marker Hf1-Indel which is developed by designing a marker according to sites with alignment gaps or missing, and finely positioning hard flesh genes of watermelons by recombinant inbred line offspring target groups after hybridization of hard-flesh watermelons PI296341-FR and cultivated watermelon 97103. Experiments prove that the molecular marker Hf1-Indel can be used for preliminarily screening watermelon flesh hardness varieties, and the purpose of molecular assisted selection is achieved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a molecular marker Hf1-Indel related to watermelon pulp hardness and an application thereof. Background technique [0002] Watermelon (Citrullus lanatus) is an important crop of Cucurbitaceae, accounting for 7% of the world's vegetable crop area, and the global annual output is about 90,000,000 tons (http: / / faostat.fao.org). my country's watermelon production and sales rank first in the world, and it is an important horticultural crop with international competitiveness and large economic growth space in my country. With the release of the whole genome sequence of watermelon, how to mine important functional genes and improve the quality of watermelon fruit is the key to molecular breeding. How to find the key genes that control the important characters of watermelon is the primary content of watermelon molecular biology research. [0003] Watermelon pulp firmness is an ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 许勇张洁张海英宫国义郭绍贵任毅
Owner 京研益农(北京)种业科技有限公司
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