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Mesenchymal stem cell culture medium

A technology for stem cells and culture medium, applied in the field of mesenchymal stem cell culture medium, can solve the problems of pollution, residue, poor cell adherence, etc., and achieve the effect of improving growth rate, reducing dead cells and increasing the number of cells

Inactive Publication Date: 2016-11-23
ANHUI HUIEN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The stem cell culture medium in the prior art often uses fetal bovine serum. Due to the use of animal-derived fetal bovine serum, the stem cell culture medium is mixed with non-human substances, resulting in residue or pollution, and the existing stem cell culture medium has cell stickers. The wall is poor, the cell proliferation rate is not ideal, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A mesenchymal stem cell culture medium proposed by the present invention comprises: DMEM medium, hydroxyethyl starch, human serum albumin, potassium chloride, sodium selenite, transferrin, glutathione, fibroblast factor, human insulin growth factor, coenzyme I, lipoic acid, vitamins, antibiotics, astragalus polysaccharide, Polygonatum polysaccharide, jujube extract.

Embodiment 2

[0019] A mesenchymal stem cell culture medium proposed by the present invention comprises: DMEM medium, hydroxyethyl starch, human serum albumin, potassium chloride, sodium selenite, transferrin, glutathione, fibroblast factor, human insulin growth factor, coenzyme I, lipoic acid, vitamins, antibiotics, astragalus polysaccharide, Polygonatum polysaccharide, jujube extract.

[0020] Wherein, the concentration of hydroxyethyl starch is 1.5g / L, the concentration of human serum albumin is 2.3g / L, the concentration of potassium chloride is 0.6g / L, and the concentration of sodium selenite is 0.5μg / L. The concentration of ferritin is 50 mg / L, the concentration of glutathione is 80 mg / L, the concentration of fibroblast cytokine is 10 μg / L, the concentration of human insulin growth factor is 25 mg / L, and the concentration of coenzyme I is 80 μg / L , the concentration of lipoic acid is 25mg / L, the concentration of vitamins is 50mg / L, the concentration of antibiotics is 8g / L, the concentr...

Embodiment 3

[0022] A mesenchymal stem cell culture medium proposed by the present invention comprises: DMEM medium, hydroxyethyl starch, human serum albumin, potassium chloride, sodium selenite, transferrin, glutathione, fibroblast factor, human insulin growth factor, coenzyme I, lipoic acid, vitamins, antibiotics, astragalus polysaccharide, Polygonatum polysaccharide, jujube extract.

[0023] Among them, the concentration of hydroxyethyl starch is 2g / L, the concentration of human serum albumin is 1.8g / L, the concentration of potassium chloride is 1.1g / L, the concentration of sodium selenite is 0.2μg / L, and the concentration of iron The concentration of protein is 100mg / L, the concentration of glutathione is 50mg / L, the concentration of fibroblast cytokine is 20μg / L, the concentration of human insulin growth factor is 15mg / L, the concentration of coenzyme I is 200μg / L, The concentration of lipoic acid is 15mg / L, the concentration of vitamins is 100mg / L, the concentration of antibiotics is...

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Abstract

The invention discloses a mesenchymal stem cell culture medium. The mesenchymal stem cell culture medium is prepared from a DMEM, hydroxyethyl starch, human serum albumin, potassium chloride, sodium selenite, transferrin, glutathione, fibroblast growth factors, human insulin-like growth factors, coenzyme I, lipoic acid, vitamins, antibiotic, astragalus polysaccharide, polygonatum polysaccharide and Chinese date extract. According to the mesenchymal stem cell culture medium, residues or pollution will not be caused as no animal source ingredient is contained, the cell wall attachment performance is good, the proliferation speed is high, the anti-pathogenic microorganism effect of the culture medium is improved, and meanwhile the cost of the culture medium is reduced.

Description

technical field [0001] The invention relates to the technical field of stem cell culture medium, in particular to a mesenchymal stem cell culture medium. Background technique [0002] Human umbilical cord mesenchymal stem cells are derived from umbilical cord Huatong jelly. They are a kind of adult stem cells with obvious plasticity and multi-directional differentiation potential. Under the regulation of different growth factors, they can differentiate into different tissue cell types derived from three germ layers. Such as adipocytes, osteoblasts, liver-like cells, cardiomyocytes, glial cells, neuron cells, etc. [0003] The stem cell culture medium in the prior art often uses fetal bovine serum. Due to the use of animal-derived fetal bovine serum, the stem cell culture medium is mixed with non-human substances, resulting in residue or pollution, and the existing stem cell culture medium has cell stickers. The wall is poor, and the cell proliferation rate is not ideal. C...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775
CPCC12N5/0668C12N2500/12C12N2500/25C12N2500/30C12N2500/34C12N2500/38C12N2500/76C12N2501/105C12N2501/90C12N2501/998
Inventor 张正亮
Owner ANHUI HUIEN BIOTECH
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