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Positive reference for STR typing

A reference, positive technology, applied in the field of forensic medicine, which can solve problems such as high cost, complicated procedures, and heavy workload

Inactive Publication Date: 2016-11-16
INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

One is that the genotype is possessed by a natural person, and the other is that the establishment of cell lines is required, which is difficult to obtain, heavy workload, complicated procedures, and high cost

Method used

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  • Positive reference for STR typing
  • Positive reference for STR typing
  • Positive reference for STR typing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Example 1, Preparation and Verification of STR Typing Positive Reference Substances

[0061] 1. Obtaining recombinant plasmids

[0062] The 26 recombinant plasmids shown in Table 1 were prepared, including 22 Y chromosome STR loci and the sex determination locus Amelogenin, with a total of 26 alleles.

[0063] According to the sequencing results, the detailed information of each recombinant plasmid is as follows (for the gene information of human genome hg38Y chromosome and human genome hg38X chromosome, please refer to the website:

[0064] http: / / genome.ucsc.edu / cgi-bin / hgTracks?hl=en chromInfoPage=&hgsid= 500042597_ceY XRRTAWoGaTHVgpCtg3gj4PilI ):

[0065] Recombinant plasmid 1: connect DNA fragment 1 and plasmid pMD18-T to obtain recombinant plasmid 1; DNA fragment 1 is to replace segment A in the DNA molecule indicated by nucleotides 4402412 to 4403492 on the human genome hg38Y chromosome with 5 '-[AGAT] 15 -3' obtained; the segment A is shown in the 4402...

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Abstract

The invention discloses a positive reference for STR typing. The positive reference for STR typing is prepared according to STR gene locus sites discovered in forensic molecular genetics. Experimental results show that the positive reference for STR typing can totally replace human genome DNA of 9948 for STR typing detection. Thus, the positive reference for STR typing can be used as a standard substance for a plurality of commercial kits and has important application value.

Description

technical field [0001] The invention relates to the technical field of forensic medicine, in particular to a positive reference substance for STR typing. Background technique [0002] Since the 1990s, STR typing technology, as the second-generation DNA typing technology, has been widely used in forensic DNA typing laboratories. The multicolor fluorescently labeled STR composite amplification product is compared with the allelic typing standard ladder to determine the genotyping of each locus. This typing result is the key to personal identification and parental relationship identification. However, due to the diversity of sources of forensic biological samples, STR typing results sometimes show missing peaks, multiple alleles detected at one locus, and even ladder-like patterns. In order to eliminate the influence of testing analysts, laboratory environmental DNA pollution and other factors on the experimental results, human genomic DNA is often used as a positive reference...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6888C12Q2600/156C12Q2600/166
Inventor 王乐马温华欧元赵兴春季安全叶健武波
Owner INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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