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Hemsleya amabilis rapid propagation medium and application thereof

A technology of culture medium and rooting medium, applied in application, horticulture, botanical equipment and methods, etc., can solve the problems of slow propagation of cuttings, long period of emergence, and small number of female parents, so as to overcome the loss of effective components. , The effect of improving the seedling rate and shortening the cultivation period

Inactive Publication Date: 2016-11-16
WUXI NANLIGONG TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The vine cutting method is limited by the season, and because the resources of the snow gall itself are not rich enough, and the number of female parents is not large, the speed of cutting propagation is slow
Snow gall seeds are rich in fat and have a thick seed coat. Before sowing, they must be stored in sand. The germination rate of snow gall seeds after sand storage can reach 20-40%. Dry storage seeds can only emerge 2 to 3 years after sowing. The germination rate Below 5%, the emergence period is long and the germination rate is low
The tissue culture of Snow Gallbladder adopts artificially prepared culture medium. Under suitable temperature and environmental conditions, fast propagation induction is carried out to Snow Gallbladder, which can shorten the cultivation time, but the existing technology is easy to cause the snow gall seedlings to Asexual variation occurs, and the rapid propagation medium in the prior art has problems such as low tissue induction rate, poor quality of induced seedlings, many weak seedlings and low seedling proliferation efficiency, and low seedling survival rate after transplanting
Experiments have shown that the content of gentamicin and genus saponin in the genus bilis cultivated by the rapid propagation medium of the prior art is quite different from that of the wild genus genus

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The invention discloses a rapid propagation medium for snow gallbladder, which is used in different stages of rapid propagation culture respectively: a callus medium, a shoot medium and a rooting medium.

[0031] The composition of the callus culture medium is: KNO 3 900mg / L, 2-amino-5-carboxypentanamide 1000mg / L, KH 2 PO 4 360mg / L, CaCl 2 2H 2 O 340mg / L, MgSO 4 ·7H 2 O 190mg / L, EDTA 150mg / L, FeSO 4 ·7H 2 O40mg / L, MnSO 4 4H 2 O 60mg / L, ZnSO 4 ·7H 2 O 20mg / L, H 3 BO 3 9mg / L, KI 8mg / L, Na 2 MoO 4 2H 2 O3mg / L, CoCl 2 ·6H 2 O 0.3mg / L, 4-iodophenoxyacetic acid 0.18mg / L, inositol 50mg / L, glycine 0.8mg / L, vitamin B1 0.65mg / L, vitamin B6 1.3mg / L, niacin 1.1mg / L, Sucrose 40g / L, agar 12g / L, kinetin KT 0.8mg / L, naphthaleneacetic acid NAA0.35mg / L, sorbitol 60g / L, biotin 0.18mg / L, pyridoxine hydrochloride 0.8mg / L, sulfur hydrochloride Amine 0.8mg / L, methylnitrosourea 1.6mg / L, indolebutyric acid 1.6mg / L, alanine 8mg / L, folic acid 8mg / L, prohexadione calcium 1.7m...

Embodiment 2

[0045] The invention discloses a rapid propagation medium for snow gallbladder, which is used in different stages of rapid propagation culture respectively: a callus medium, a shoot medium and a rooting medium.

[0046] The composition of described callus culture medium is: KNO 3 800mg / L, 2-amino-5-carboxypentanamide 800mg / L, KH 2 PO 4 300mg / L, CaCl 2 2H 2 O 320mg / L, MgSO 4 ·7H 2 O 180mg / L, EDTA 120mg / L, FeSO 4 ·7H 2 O 30mg / L, MnSO 4 4H 2 O 50mg / L, ZnSO 4 ·7H 2 O 15mg / L, H 3 BO 3 8mg / L, KI 5mg / L, Na 2 MoO 4 2H 2 O 1mg / L, CoCl 2 ·6H 2 O 0.2mg / L, 4-iodophenoxyacetic acid 0.15mg / L, inositol 30mg / L, glycine 0.5mg / L, vitamin B1 0.5mg / L, vitamin B6 1.0mg / L, niacin 1.0mg / L, Sucrose 30g / L, agar 10g / L, kinetin KT0.5mg / L, naphthaleneacetic acid NAA0.3mg / L, sorbitol 50g / L, biotin 0.15mg / L, pyridoxine hydrochloride 0.5mg / L, sulfur hydrochloride Amine 0.5mg / L, methylnitrosourea 1.5mg / L, indole butyric acid 1.5mg / L, alanine 5mg / L, folic acid 5mg / L, prohexadione calcium...

Embodiment 3

[0059] The invention discloses a rapid propagation medium for snow gallbladder, which is used in different stages of rapid propagation culture respectively: a callus medium, a shoot medium and a rooting medium.

[0060] The composition of described callus culture medium is: KNO 3 1300mg / L, 2-amino-5-carboxypentanamide 1200mg / L, KH 2 PO 4 450mg / L, CaCl 2 2H 2 O 350mg / L, MgSO 4 ·7H 2 O 240mg / L, EDTA 180mg / L, FeSO 4 ·7H 2O 50mg / L, MnSO 4 4H 2 O 80mg / L, ZnSO 4 ·7H 2 O 30mg / L, H 3 BO 3 10mg / L, KI 10mg / L, Na 2 MoO 4 2H 2 O 5mg / L, CoCl 2 ·6H 2 O 0.5mg / L, 4-iodophenoxyacetic acid 0.20mg / L, inositol 80mg / L, glycine 1.0mg / L, vitamin B1 0.75mg / L, vitamin B6 1.5mg / L, niacin 1.5mg / L, Sucrose 50g / L, agar 15g / L, kinetin KT 1.0mg / L, naphthaleneacetic acid NAA 0.4mg / L, sorbitol 70g / L, biotin 0.20mg / L, pyridoxine hydrochloride 1.0mg / L, sulfur hydrochloride Amine 1.0mg / L, methylnitrosourea 2.0mg / L, indolebutyric acid 2.0mg / L, alanine 10mg / L, folic acid 10mg / L, prohexadione ...

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PUM

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Abstract

The invention discloses a hemsleya amabilis rapid propagation medium and application thereof. The hemsleya amabilis rapid propagation medium comprises a callus medium, a tender shoot medium and a rooting medium which are used in different stages of hemsleya amabilis rapid propagation culture respectively. The hemsleya amabilis rapid propagation medium provides scientific and reasonable nutrients in the inducing culture process of hemsleya amabilis callus, the seedling rate of seedlings is increased, the period of tissue inducing culture is shortened, the medicinal effect of wild hemsleya amabilis is kept, and the hemsleya amabilis rapid propagation medium has application prospects in the aspects of hemsleya amabilis wild resource preservation, protection, development and utilization and the like.

Description

technical field [0001] The invention relates to a culture medium and its application, in particular to a snow gall rapid propagation medium and its application, and belongs to the field of agricultural high-tech. Background technique [0002] Snow gall is a plant of the genus Snow gall of Cucurbitaceae. According to current knowledge, there are more than 20 species of this genus, mainly produced in Southwest China. It is bitter and cold in nature, less used in traditional medicine, and rich in wild resources. However, with the development of medicine, studies have found that snow gallbladder has the effects of clearing heat and detoxifying, reducing inflammation and relieving pain. Commonly used for gastric pain, ulcer disease, upper respiratory tract infection, bronchitis, pneumonia, bacillary dysentery, enteritis, urinary tract infection, sepsis and other infections. In recent years, Xuedansu Tablets, Xuedan Weichang Pills, Xuedansu Capsules, Xuedanjiedu Pills and other ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 许建中林少会孟剑华
Owner WUXI NANLIGONG TECH DEV
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