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EST-SSR primer group for identifying dendrobium officinale Kimura et migo and dendrobium falconeri hook, preparation method of EST-SSR primer group and application of EST-SSR primer group in species identification

A Dendrobium candidum and primer set technology, applied in biochemical equipment and methods, recombinant DNA technology, microbial measurement/inspection, etc., to achieve the effect of reducing errors or deviations

Active Publication Date: 2016-11-09
江西新灵倍康食品发展有限公司
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Problems solved by technology

But so far, EST-SSR markers of Dendrobium officinale are rarely reported

Method used

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  • EST-SSR primer group for identifying dendrobium officinale Kimura et migo and dendrobium falconeri hook, preparation method of EST-SSR primer group and application of EST-SSR primer group in species identification
  • EST-SSR primer group for identifying dendrobium officinale Kimura et migo and dendrobium falconeri hook, preparation method of EST-SSR primer group and application of EST-SSR primer group in species identification
  • EST-SSR primer group for identifying dendrobium officinale Kimura et migo and dendrobium falconeri hook, preparation method of EST-SSR primer group and application of EST-SSR primer group in species identification

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Embodiment

[0029] The primer set sequence of the present invention is:

[0030] DN13 F:ATTTGATTGGTTCTGCTCATCC R:TTCTTGTTCTCTTCCAGCTTCC

[0031] DN58 F: CTAATGCAGGTTCTCCTCACCT R: GTTCAGCTCATCTCCTTGGATT

[0032] DN65 F:GATAAGGGGAAGGAGGAGAAGA R:AATCTATGCCAATCACTCCACA

[0033] DN67 F: GCACACATCCACTCACCCT R: GGGTATAGAGAGAAATGCTGCG

[0034] DN99 F: GGCACAGGTACTAGCAACAACA R: AGGTGATGGCAAAGTTCCAA.

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Abstract

The invention discloses an EST-SSR primer group for identifying dendrobium officinale Kimura et migo and dendrobium falconeri hook. The primer group is characterized by consisting of five pairs of primers; and specifically, sequence lists of the primers are shown as follows: DN13 F:ATTTGATTGGTTCTGCTCATCC R:TTCTTGTTCTCTTCCAGCTTCC, DN58 F:CTAATGCAGGTTCTCCTCACCT R:GTTCAGCTCATCTCCTTGGATT, DN65 F:GATAAGGGGAAGGAGGAGAAGA R:AATCTATGCCAATCACTCCACA, DN67 F:GCACACATCCACTCACCCT R:GGGTATAGAGAGAAATGCTGCG and DN99 F:GGCACAGGTACTAGCAACAACA R:AGGTGATGGCAAAGTTCCAA. The primer group has the advantages of reducing biases or errors of strip interpretation, and simultaneously reflecting group or individual genetic variation and bringing about benefits for researches on functional genes.

Description

Technical field [0001] The invention relates to the technical field of Dendrobium officinale, in particular to an EST-SSR primer set for identifying Dendrobium officinale and Dendrobium candidum, a preparation method and its application in species identification. Background technique [0002] Dendrobium officinale (scientific name: Dendrobium officinale Kimura et Migo), also known as: black knotweed, Dendrobium officinale rhyme, and purple wormwood. It is a perennial epiphytic herb of Orchidaceae. The stem is erect, cylindrical, 9-35 cm long, 2-4 mm thick, with sepals and petals yellow-green, nearly similar, oblong-lanceolate, about 1.8 cm long and 4 to 5 mm wide, and flowering from March to June. Mainly distributed in China's Anhui, Zhejiang, Fujian and other places, its stem is used as medicine, which is a tonic medicine in tonic medicine. Among the existing 76 species of Dendrobium plants in China, there are more than 40 medicinal plants. Its pharmacology is mainly to streng...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 付涛何月秋胡仲义王志龙
Owner 江西新灵倍康食品发展有限公司
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