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Preparation method of phosphatidylserine

A technology of phosphatidylserine and serine, which is applied in the direction of fermentation, etc., can solve the problems of low purity, high cost of raw materials, and complicated extraction methods, and achieve the effect of high purity and increased application range

Inactive Publication Date: 2016-11-09
WUHU FOMAN BIOPHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the above-mentioned prior art, the purpose of the present invention is to overcome the less content of phosphatidylserine in plants in the prior art, so that its extraction requires a large amount of raw materials, and the purity is not high, and the extraction from animal cells, Often only animal brain cells can be used for extraction, not only the cost of raw materials is high, but also the extraction method is complicated, so a method for preparing phosphatidylserine with relatively simple preparation method and high purity of phosphatidylserine is provided

Method used

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preparation example Construction

[0015] The present invention provides a preparation method of phosphatidylserine, wherein the preparation method includes:

[0016] 1) Mix soybean lecithin and water to prepare mixture M1;

[0017] 2) Add L-serine and phospholipase D to the mixture M1 and mix to obtain crude phosphatidylserine;

[0018] 3) Adding solvent extraction to the crude phosphatidylserine prepared above to prepare a semi-finished phosphatidylserine;

[0019] 4) Pass the semi-finished phosphatidylserine prepared above through a silica gel column and an ion exchange resin in sequence to obtain phosphatidylserine; wherein,

[0020] The solvent is composed of chloroform, ethanol, ether and water.

[0021] In the above design, a crude product of phosphatidylserine is prepared by mixing soybean lecithin with water, then adding L-serine and phospholipase D to react, and then using a solvent containing chloroform, ethanol, ether and water in the crude product of phosphatidylserine After extraction, it is filtered throug...

Embodiment 1

[0029] Mix 150g of water, 30g of chloroform, 45g of ethanol and 15g of ether to prepare a solvent; mix 30g of soybean lecithin and 100g of water to prepare a mixture M1; add 15g of L-serine and 1g of phospholipase D at 25°C to the mixture M1 Mix under conditions for 25 hours to obtain crude phosphatidylserine; add 200g solvent to the crude phosphatidylserine prepared above, and repeat the extraction process twice to obtain a semi-finished phosphatidylserine; The semi-finished serine passes through a silica gel column and a cation exchange resin in sequence to prepare phosphatidylserine A1.

Embodiment 2

[0031] Mix 200 g of water, 160 g of chloroform, 120 g of ethanol and 60 g of ether to prepare a solvent; mix 50 g of soybean lecithin and 100 g of water to prepare a mixture M1; add 20 g of L-serine and 5 g of phospholipase D at 30°C to the mixture M1 Mix under conditions for 35 hours to obtain crude phosphatidylserine; add 500g solvent to the crude phosphatidylserine prepared above, and repeat the extraction process 4 times to obtain a semi-finished phosphatidylserine; The semi-finished serine passes through a silica gel column and a cation exchange resin in sequence to prepare phosphatidylserine A2.

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Abstract

The invention discloses a preparation method of phosphatidylserine. The preparation method comprises the following steps: (1) mixing soya bean lecithin and water, thus preparing a mixture M1; (2) adding L-serine and phospholipase D into the mixture M1, and mixing L-serine, phospholipase D and the M1, thus preparing a phosphatidylserine crude product; (3) adding a solvent into the prepared phosphatidylserine crude product for extraction, thus preparing a phosphatidylserine semi-finished product; and (4) enabling the prepared phosphatidylserine semi-finished product to sequentially pass through a silicagel column and an ion exchange resin, thus preparing phosphatidylserine, wherein the solvent is composed of chloroform, ethanol, diethyl ether and water. According to the design, soya bean lecithin is mixed with water firstly, then L-serine and phospholipase D are added for carrying out reaction, then the solvent containing chloroform, ethanol, diethyl ether and water is adopted for carrying out extraction, and the obtained phosphatidylserine semi-finished product is filtered by the silicagel column and the ion exchange resin, the operation is simple and convenient, the prepared phosphatidylserine is high in purity, and the application range of phosphatidylserine is greatly expanded.

Description

Technical field [0001] The present invention relates to the production and preparation field of phosphatidylserine, in particular to a preparation method of phosphatidylserine. Background technique [0002] Phosphatidylserine, also known as compound nervonic acid, is an active substance in cell membranes, especially in brain cells. Its main function is to improve nerve cell function, regulate nerve impulse conduction, and enhance brain memory function. Therefore, it plays an extremely important role in the human body. [0003] Nowadays, the preparation of phosphatidylserine is mainly the extraction method, but the content of phosphatidylserine in plants is often small, so that its extraction requires a large amount of raw materials, and the purity is not high, and the extraction from animal cells is often only Can use animal brain cells for extraction, not only the raw material cost is high, but also the extraction method is complicated. [0004] Therefore, it is an urgent problem ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64
CPCC12P7/6481C12P13/06
Inventor 苏福男
Owner WUHU FOMAN BIOPHARMA CO LTD
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