Preparation method of catechin nanoparticles reducing aflatoxin bioavailability

A technology of aflatoxin and catechin is applied in the field of preparation of catechin nanoparticles, which can solve the impact, and at the same time, there are also research reports and problems such as reducing the bioavailability of anti-aflatoxin

Active Publication Date: 2018-12-21
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the preparation of catechin microcapsules and the anti-injury effect by reducing the bioavailability of anti-aflatoxin
Although there are literatures reporting the effects of different concentrations of green tea and tea residues on the effect of aflatoxin B1 on liver cancer in rats, and there are also studies reporting the chemopreventive effect of tea polyphenols on the formation of tree shrew liver cancer, but the above studies are only from the perspective of liver damage. To study the protective or intervention effect of tea polyphenols on aflatoxin damage, but from the perspective of new dosage forms and reducing the bioavailability of aflatoxin to study the protective function of aflatoxin, there is no report

Method used

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  • Preparation method of catechin nanoparticles reducing aflatoxin bioavailability
  • Preparation method of catechin nanoparticles reducing aflatoxin bioavailability
  • Preparation method of catechin nanoparticles reducing aflatoxin bioavailability

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Embodiment 1, a kind of preparation method of the catechin nanoparticle that can reduce aflatoxin bioavailability, carries out following steps successively:

[0069] 1) Add 0.5g of chitosan to a 500mL round-bottomed flask, add water to make the volume to 100mL, stir magnetically at 25°C for 30 minutes, rotate at 900r / min, and adjust the pH to 4.5;

[0070] 2), accurately preparing 20 mL of sodium polyphosphate solution with a mass concentration of 1.5%, and using the sodium polyphosphate solution to prepare catechin-sodium polyphosphate solution with a catechin concentration of 60 mg / mL;

[0071] 3), the catechin--sodium polyphosphate solution obtained in step 2) is all slowly dripped into the round bottom flask in 1) drop by drop, and the drop time is 10 minutes (the drop rate is about 40 drops / minute);

[0072]The obtained reaction system was continuously stirred at 25° C. for 50 minutes (rotating speed: 400 r / min) to obtain a catechin nanoparticle suspension.

Embodiment 2

[0073] Embodiment 2, a kind of preparation method of the catechin nanoparticle that can reduce the bioavailability of aflatoxin, carries out the following steps successively:

[0074] 1) Add 0.2g of chitosan to a 500mL round-bottomed flask, add water to make up to 100mL, stir magnetically at 25°C for 30 minutes, rotate at 500r / min, and adjust the pH to 3.0;

[0075] 2), accurately preparing 20 mL of sodium polyphosphate solution with a mass concentration of 1.0%, and using the sodium polyphosphate solution to prepare catechin-sodium polyphosphate solution with a catechin concentration of 60 mg / mL;

[0076] 3), the catechin-sodium polyphosphate solution obtained in step 2) is all slowly added dropwise evenly to the round bottom flask in 1), and the dropping time is 10 minutes;

[0077] The obtained reaction system was continuously stirred at 25° C. for 10 minutes to obtain a catechin nanoparticle suspension.

Embodiment 3

[0078] Embodiment 3, a kind of preparation method of the catechin nanoparticle that can reduce the bioavailability of aflatoxin, carries out the following steps successively:

[0079] 1) Add 1.5g of chitosan to a 500mL round-bottomed flask, add water to make the volume to 100mL, and perform magnetic stirring at 25°C for 60 minutes at a speed of 1500r / min, while adjusting the pH to 7.0;

[0080] 2), accurately preparing 20 mL of sodium polyphosphate solution with a mass concentration of 2.5%, and then preparing a catechin-sodium polyphosphate solution with a catechin concentration of 60 mg / mL with the sodium polyphosphate solution;

[0081] 3), the catechin-sodium polyphosphate solution obtained in step 2) is all slowly added dropwise evenly to the round bottom flask in 1), and the dropping time is 5 minutes;

[0082] The obtained reaction system was continuously stirred at 25° C. for 80 minutes to obtain a suspension of catechin nanoparticles.

[0083] Experiment 1. Detection...

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Abstract

The invention discloses a preparation method of catechin nanoparticles capable of reducing aflatoxin bioavailability. The preparation method comprises the following steps: 1) adding water to 0.4-0.6g of chitosan and setting constant volume to 100ml, conducting magnetic stirring and regulating pH to 4.0-5.0; 2) adding catechin to 20mL of a sodium polyphosphate solution which is 1.2-1.8% in mass concentration, so that a catechin-sodium polyphosphate solution that the concentration of the catechin is 60-65mg / mL is prepared; and 3) completely adding the obtained catechin-sodium polyphosphate solution to the material obtained in the step 1), and continuing to stir an obtained reaction system at 20-30 DEG C for 30-50min, so that a catechin nanoparticle suspension is obtained.

Description

technical field [0001] Catechin is the most important natural active ingredient in tea, which has various pharmacological and health-care activities. The invention relates to a preparation method of catechin nanoparticles capable of reducing the bioavailability of aflatoxin. Background technique [0002] Tea Polyphenols (Tea Polyphenols, TP) is the general term for polyphenols and their derivatives in tea, mainly composed of catechins, flavonoids, anthocyanins and phenolic acids, among which catechins account for the majority of tea polyphenols. Total 60%-90%. Catechin has strong biological activity. A large number of studies have shown that catechin has various health care and pharmacological effects such as anti-oxidation, scavenging free radicals, anti-cardiovascular disease, antibacterial and anti-inflammatory. The chairman of the American Medical Foundation believes: "Tea polyphenols (catechins) are compounds that have a huge effect on human health in the 21st century...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/353A61K9/14A61P39/06A61P9/00A61P29/00
CPCA61K9/146A61K31/353
Inventor 肖英平杨华钱鸣蓉李锐吉小凤王小骊汪建妹
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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