Method for inducing dendrocalamus latiflorus stem apex calluses and obtaining regeneration plants

Abstract

The invention discloses a method for inducing dendrocalamus latiflorus stem apex calluses and obtaining regeneration plants and belongs to the field of rapid propagation of forests. The method comprises the following steps: selecting explants, carrying out induction culture and propagation culture on the calluses, inducing and germinating embryoids, rooting, strengthening seedlings, hardening the seedlings and transplanting the seedlings into soil. Through an induction medium, a propagation medium, a germination induction medium and a rooting medium, dendrocalamus latiflorus is high in differentiation efficiency, high in propagation coefficient and high in rooting rate; the needed growth time of plants is short; the cultivated plants are high in adaptability; the seedlings are strong, tall, straight, regular and consistent, and are good in growth vigor; the culture process of the seedlings is greatly shortened; a large amount of cost can be reduced; a callus induction system taking the nutritive tissues of the dendrocalamus latiflorus as sources is built; the regeneration plants are successfully obtained; a technical means is supplied to rapid propagation of the dendrocalamus latiflorus seedlings; a platform is provided for building a dendrocalamus latiflorus transgenosis system in the future.

Description

technical field [0001] The invention belongs to the field of rapid propagation of forest trees, and in particular relates to a method for inducing callus at the stem end of Mazak basilica and obtaining regenerated plants. Background technique [0002] Bamboo is a subgenus of Bamboo, Bamboo subfamily of Poaceae. It is naturally distributed in Fujian, Taiwan, Guangdong, Guangxi, Guizhou, Yunnan and other parts. High economic value and social value. There are relatively few studies on the tissue culture of Bamboo bamboo, and currently the anthers and embryos are the main explants. In 1987, Yeh et al. successfully induced the callus of Bamboo japonica and obtained regenerated plants from the seed embryo of Bamboo bazaar . In 1990, Tsay et al. used the anthers of Bamboo bamboo to induce calluses to obtain regenerated plants, but the haploid plants obtained by this method died within half a year after transplanting, which shows that this method is less feasible in production. ...

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Problems solved by technology

In 1987, Yeh et al. successfully induced the callus of Bamboo japonica and obtained regenerated plants from the seed embryo of Bamboo bazaar

In 1990, Tsay et al. used the anthers of Bamboo bamboo to induce calluses to obtain regenerated plants, but the haploid plants obtained by this method died within half a year after transplanting, which shows that this method is less feasible in production. ; At the same time, this system has great limitations on the acquisition of bamboo explants. For example, the flowering cycle of bamboo is very long and rarely blooms, so it is not easy to obtain anthers; theoretically, the regenerated plants cultivated by anthers Changing from diploid to haploid has unpredictable effects on the later development of plants

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