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A kind of transplanting method of kiwifruit virus-free tissue culture seedling

A technology for tissue culture seedlings and kiwifruit, which is applied in horticultural methods, botanical equipment and methods, cultivation, etc., can solve the problems of low plant survival rate, labor and material resources consumption, and easy damage, so as to reduce the spread of soil-borne diseases, Ensure the safety of the growing environment and the effect of ensuring nutritional requirements

Active Publication Date: 2018-02-23
武汉植物园园艺中心有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] 1. Kiwi fruit tissue culture seedlings grow indoors for a short time, the plants themselves are not strong enough, and their stress resistance is poor;
[0005] 2. Kiwi fruit tissue culture seedlings take root slowly, and are easily damaged in the later transplanting process. After transplanting, the rooting speed and the ratio of regenerated roots are low, resulting in a low survival rate after transplanting;
[0006] 3. The transplanting environment of kiwifruit tissue culture seedlings is simple, and there is no isolation treatment from the outside world, and the tissue culture seedlings are easy to be repeatedly infected with the virus;
[0007] 4. After the tissue-cultured seedlings have been domesticated and transplanted and survived, they must be artificially planted before sales. This process consumes manpower and material resources, and the survival rate of the plants after artificial planting is low.

Method used

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  • A kind of transplanting method of kiwifruit virus-free tissue culture seedling

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Embodiment 2

[0058] ‘Donghong’ virus-free tissue culture seedling transplanting method:

[0059] ① Treatment of tissue culture seedlings

[0060] Adjust the photoperiod of 2-3 weeks old tissue cultured seedlings to 12 hours of light and 12 hours of darkness; at the same time, the light intensity during the light is increased to 3000-5000 lux;

[0061] ②Cultivated seedling preparation

[0062] 10-15 days after the inoculation of the tissue culture seedlings, take out the tissue culture seedlings in the tissue culture bottle, and rinse the basal medium with clean water until it is washed. Immerse the base of the plant in 25 mg / L naphthalene acetic acid solution for 1 to 2 hours, and spray water evenly on the leaves to keep the stems and leaves moist.

[0063] ③Transplanting

[0064]3-5 days before transplanting, sterilize the nutrient bowl and seedling nutrient soil with formaldehyde, and 1 day before transplanting, ventilate the nutrient bowl and seedling nutrient soil until the residual...

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Abstract

The invention discloses a kiwi fruit detoxification tissue culture seedling transplanting method, relating to the technical field of fruit tree facility cultivation. The method comprises the following steps: (1) tissue culture seedling treating: adjusting the photoperiod of tissue culture seedlings with a seedling age of 3-4 weeks to be 12 hours with illumination and 12 hours in darkness, and meanwhile increasing the illumination intensity during illumination to 3000-5000lux; (2) cultivation seedling preparation: after treating the tissue culture seedlings for 10-15 days, taking out the tissue culture seedlings in a tissue culture bottle, and flushing a stem base culture medium with clear water, until the stem base culture medium is cleaned thoroughly; soaking stem bases into rooting solution, uniformly spraying water on leaf surfaces, and keeping stem leaves wet; (3) transplanting; (4) outdoor seedling hardening. According to the kiwi fruit detoxification tissue culture seedling transplanting method, the illumination time in a later rapid propagation period of the tissue culture seedlings is increased, the illumination intensity is enhanced, the plant stress resistance is enhanced, and the survival rate is ensured; a hole transplanting link is omitted, and manpower and material resources are saved; greenhouse facilities are adopted for cultivation, and the quality of the kiwi fruit tissue culture seedlings is ensured; a soil-free cultivation mode is adopted, the soil-borne disease propagation is reduced, and the nutrition requirements in a kiwi fruit seedling period are met.

Description

technical field [0001] The invention relates to the technical field of facility cultivation of fruit trees, in particular to a method for transplanting kiwi fruit virus-free tissue-cultured seedlings. Background technique [0002] Kiwifruit (Lycium chinense) is a plant of the genus Actinidia in the family Actinidia. It is rich in kiwifruit proteoglycan, VC, phosphorus, iron and other nutrients. At present, kiwifruit propagation techniques are mostly cutting and grafting in production, which can retain the excellent traits of the mother, but also retain the plant viruses in the mother; the accumulation of viruses in kiwifruit increases from generation to generation, and viral diseases continue to affect the yield of kiwifruit. Fruit quality is declining year by year. [0003] Existing technology The rapid propagation of kiwifruit detoxification tissue culture is divided into five stages: (1) obtaining sterile detoxification materials; (2) primary culture of kiwifruit tissue ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G17/00A01G31/00A01G7/04
CPCA01G7/04A01G17/005A01G31/00Y02P60/14
Inventor 吕海燕钟彩虹
Owner 武汉植物园园艺中心有限公司
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