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PVT1 siRNA-1055 for inhibiting blood tumor cell proliferation and application thereof

A technology for hematological tumors and cell proliferation, applied in DNA/RNA fragments, anti-tumor drugs, recombinant DNA technology, etc., to achieve the effect of inhibiting proliferation and huge market potential

Active Publication Date: 2016-10-26
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Existing studies have shown that PVT1 is associated with the occurrence of hematological malignancies, but there is currently no evidence that PVT1 siRNA can be used in tumors, especially hematological malignancies for treatment and / or prevention

Method used

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  • PVT1 siRNA-1055 for inhibiting blood tumor cell proliferation and application thereof
  • PVT1 siRNA-1055 for inhibiting blood tumor cell proliferation and application thereof
  • PVT1 siRNA-1055 for inhibiting blood tumor cell proliferation and application thereof

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Embodiment 1

[0025] 1. Experimental materials and methods

[0026] (1) Design and synthesis of PVT1 siRNA

[0027] According to the design tool software provided by Ambion on the Internet (see www.ambion.com / techlib / misc / siRNA finder.html), four siRNA sequences were designed for PVT1 RNA, named PVT1 siRNA-1055, PVT1 siRNA-845, PVT1 respectively siRNA-176 and PVT1 siRNA-54, while designing an irrelevant negative control sequence (negative control, NC) siRNA as a control, were synthesized by Shanghai Gemma Gene Chemical Technology Co., Ltd.

[0028] The sequence of PVT1 siRNA-1055 is as follows:

[0029] Sense strand: 5′-GCUUCUCCUGUUGCUGCUATT-3′;

[0030] Antisense strand: 5′-UAGCAGCAACAGGAGAAGCTT-3′;

[0031] The sequence of PVT1 siRNA-845 is as follows:

[0032] Sense strand: 5′-CCUGUUACACCUGGGAUUUTT-3′;

[0033] Antisense strand: 5′-AAAUCCCAGGUGUAACAGGTT-3′;

[0034] The sequence of PVT1 siRNA-176 is as follows:

[0035] Sense strand: 5'-GCUGAAUGCCUCAUGGAUUTT-3';

[0036] Antisens...

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Abstract

The invention discloses PVT1 siRNA-1055 for inhibiting blood tumor cell proliferation and an application thereof and relates to the fields of siRNA and application thereof. The PVT1 siRNA-1055 in the invention can high-effectively inhibit expression of PVT1 and effectively inhibit proliferation of the blood tumor cells, and has important significance on development of novel anti-blood tumor gene medicines and improvement of therapy effect of blood tumors. As deterioration of living environment of human, morbidity and mortality of blood tumor (such as leukemia and lymphoma) are higher and higher, wherein the blood tumor has become one of main neoplastic diseases that influence health and life of people. Almost all blood tumors have PVT1 expression increase, wherein the PVT1 has the functions as a cancer gene, so that targeted PVT1 therapy has a universal significance, is suitable for almost all blood tumors, and has a huge market potential.

Description

technical field [0001] The present invention relates to the field of siRNA and its application, in particular to a PVT1 siRNA-1055 that inhibits the proliferation of hematological tumor cells and its application in the preparation of drugs for treating and / or preventing hematological tumors. Background technique [0002] RNAi technology is a new way to inhibit gene expression with high efficiency and high specificity. In vivo and in vitro studies have shown that RNAi technology has better application prospects than antisense nucleic acid in cancer gene therapy. A large number of studies have shown that inhibiting the expression of tumor-related genes by RNAi in vitro can inhibit the proliferation and growth of tumor cells, induce apoptosis, and increase the sensitivity of tumor cells to drugs. [0003] Long non-coding RNAs (lncRNAs) are RNAs with a length of more than 200 nucleotides that do not encode proteins and play an important role in regulating cell proliferation and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113A61K31/713A61P35/00A61P35/02
CPCC12N15/1135C12N2310/14
Inventor 何冬梅郑婵丽陈盛亭李扬秋
Owner JINAN UNIVERSITY
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