Production line for culturing plant stem cells on large scale

Abstract

The invention discloses a production line for culturing plant stem cells on a large scale. The production line comprises a tray washing and drying line and a medium filling, freezing and inoculating line; the tray washing and drying line comprises a first conveying belt, a washing bin, a water blowing bin and a drying bin, and the first conveying belt sequentially penetrates through the washing bin, the water blowing bin and the drying bin; the medium filling, freezing and inoculating line comprises a second conveying belt, an automatic filling device, a freezing bin and an inoculating machine, the automatic filling device, the freezing bin and the inoculating machine are sequentially arranged in the conveying direction of the second conveying belt, an efficient filter, a draught fan and a condenser are sequentially arranged in the freezing bin, the inoculating machine is provided with a hopper, and the hopper is arranged above the second conveying belt. According to the production line, the thought of conventional liquid suspension culture is avoided during factory-like culture of the plant stem cells, and a simpler and more efficient solid culture technical system is developed.

Description

technical field [0001] The invention relates to the technical field of cell culture systems, in particular to a production line for large-scale culture of plant stem cells. Background technique [0002] Liquid suspension culture in fermenters is usually efficient and practical for industrial production of microorganisms, which has been confirmed by a large number of production cases. The volume of plant cells is dozens of times larger than that of microorganisms, and they are more sensitive to various stresses in the suspension culture process; each time the volume of liquid suspension culture is enlarged, new culture conditions need to be studied, and the final culture volume that can be enlarged is unknown. The process leading to the pilot test will be very long, expensive and uncertain of the results. This has been confirmed in the suspension culture of many plant cells in the past. Even if the pilot test is successful, the investment cost of purchasing a fermenter in l...

AI Technical Summary

Problems solved by technology

Even if the pilot test is successful, the investment cost of purchasing fermentation tanks in large-scale production is very high, the production process is more complicated, consumes more electric energy and produces a large amount of waste water

The proliferation cycle of different plant cells is as long as 3-10 days, and a complete fermentation culture cycle is usually as long as 2-4 months, which leads to only a few production cycles per year, and the final output is limited

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