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Multidimensional gas chromatography-tandem mass spectrometry analytical method for nicotine optical isomer in electronic cigarette smoke solution

A multi-dimensional gas chromatography, e-cigarette liquid technology, applied in the direction of analysis materials, material separation, measurement devices, etc., can solve the problems of low concentration, unable to meet the needs of nicotine optical isomer analysis, etc., and achieves low detection limit , good recovery rate and repeatability, easy and fast operation

Inactive Publication Date: 2016-10-12
CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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Problems solved by technology

Considering that most of the nicotine in e-cigarette liquids comes from tobacco extracts, and the ratio of R-(+)-nicotine is about 0.2-0.3%, this patented method cannot meet the requirements of most e-cigarette liquids. The need for the analysis of nicotine optical isomers
The content of nicotine in e-cigarette liquids varies widely, and merchants add different concentrations of nicotine to e-cigarette liquids according to market needs. At the same time, traces of nicotine can also be detected in some e-cigarette liquids that claim to contain no nicotine. exists, but presents a challenge for the chiral analysis of nicotine due to its low concentration

Method used

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  • Multidimensional gas chromatography-tandem mass spectrometry analytical method for nicotine optical isomer in electronic cigarette smoke solution
  • Multidimensional gas chromatography-tandem mass spectrometry analytical method for nicotine optical isomer in electronic cigarette smoke solution
  • Multidimensional gas chromatography-tandem mass spectrometry analytical method for nicotine optical isomer in electronic cigarette smoke solution

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Embodiment Construction

[0032] The present invention is further described by the following specific examples, but does not limit the present invention.

[0033] 1. Instruments and Reagents

[0034] MDGCMS-QP2010Ultra Multidimensional GC / MS (Shimadzu Corporation, Japan), Autosampler AOC-5000 (Shimadzu Corporation, Japan), automatic solid-phase microextraction fiber sampling needle, 65 μm PDMS / DVB (polydimethylsiloxane) Oxane / divinylbenzene) stationary phase extraction fiber head (Supelco, USA), Rtx-5MS column (30 m × 0.25 mm × 0.25 μm, Shimadzu Corporation, Japan), InertCap CHIRAMIX column (30 m × 0.25 mm × 0.25 μm, Shimadzu Corporation, Japan), AE163 electronic balance (sensitivity: 0.0001 g, Mettler, Switzerland), HY-8 speed regulating oscillator (Changzhou Guohua Electric Co., Ltd.). Methanol (chromatographically pure), S-(-)-nicotine standard (CAS: 54-11-5), R-(+)-nicotine standard (CAS: 25162-00-9).

[0035] 2. Preparation of standard solutions

[0036] Weigh about 100.0 mg of S-(-)-nicotine s...

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Abstract

The invention discloses a multidimensional gas chromatography-tandem mass spectrometry analytical method for a nicotine optical isomer in an electronic cigarette smoke solution. The method is characterized in that a common cigarette smoke solution is diluted by methanol and is directly subjected to multidimensional gas chromatography-tandem mass spectrometry detection, the electronic cigarette smoke solution without nicotine or low nicotine content is diluted by methanol, then is subjected to automatic solid-phase micro-extraction, and is subjected to the multidimensional gas chromatography-tandem mass spectrometry detection; and a peak area normalization method is used for quantitative determination of the proportion of S-(-)-nicotine and R-(+)-nicotine in the electronic cigarette smoke solution. The detection method has the advantages that sample processing is simple and fast, detection limit of the method is low, sensitivity is high, precision is good, and the analysis of a sample of the electronic cigarette smoke solution can be satisfied.

Description

technical field [0001] The invention relates to a method for determining chemical components of tobacco, in particular to a method for analyzing the optical isomers of nicotine in electronic cigarette liquid by multi-dimensional gas-phase gas chromatography-mass spectrometry. Background technique [0002] Nicotine is the most important pharmacological component of tobacco and one of the most important reasons why people consume tobacco. The nicotine molecule contains a chiral center, the 2-position carbon atom on the tetrahydropyrrole ring, so nicotine has two enantiomers: S-(-)-nicotine and R-(+)-nicotine. In tobacco, the two enantiomers have completely different contents, metabolic mechanisms and physiological properties. The content of S-(-)-nicotine in tobacco is much higher than that of R-(+)-nicotine, and R-(+)-nicotine accounts for 0.2-0.3% of the total nicotine in tobacco. In the process of isomer turnover, the content of R-(+)-nicotine in cigarette smoke can reach...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/884
Inventor 付亚宁韩书磊刘彤陈欢侯宏卫胡清源
Owner CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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