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Kit for testing alpha-L-fucosidase

A fucosidase and fucosidase technology, which is applied in the fields of medicine and biochemistry, can solve the problems of inaccurate detection, complicated operation and high cost, and achieve the effects of ensuring accuracy, accurate detection and maintaining activity.

Inactive Publication Date: 2016-10-12
ANHUI IPROCOM BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Aiming at the defects of complicated operation, long time-consuming, high cost and inaccurate detection, the present invention provides a kit for measuring α-L-fucosidase

Method used

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  • Kit for testing alpha-L-fucosidase

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Embodiment Construction

[0039] Specific embodiments of the present invention will be described in detail below. It should be understood that the specific embodiments described here are only used to illustrate and explain the present invention, and are not intended to limit the present invention.

[0040] The invention discloses a kit for measuring α-L-fucosidase. The kit is composed of a single reagent, and the components and corresponding contents are as follows:

[0041] 2-hydroxypropane-1,2,3-tricarboxylic acid 30~90 mmol / L;

[0042] Sodium chloride 60~100 mmol / L;

[0043] Sodium azide 0.1~0.6 g / L;

[0044] EDTA 12~26mmol / L;

[0045] 2-Chloro-4-nitrobenzene-α-L-fucoside 18~72 mmol / L;

[0046] Its solvent is purified water.

[0047] Preferably, Ming also discloses the preparation steps of a single reagent in the above kit, and the specific preparation steps are as follows:

[0048] (1) Dissolve 2-hydroxypropane-1,2,3-tricarboxylic acid in purified water, stir evenly, and prepare buffer;

[0...

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Abstract

The invention relates to the technical fields of medicine and biochemistry, in particular to a kit for measuring α-L-fucosidase. The existing methods for detecting α-L-fucosidase have defects such as high detection cost, complicated and time-consuming operation, and only qualitative or semi-quantitative detection. The present invention provides a kit for measuring α-L-fucosidase, which can solve the above problems. The technical scheme of the present invention adopts the following kit reagent component formula: 2-hydroxypropane-1,2,3-tricarboxylic acid 30~90 mmol / L, sodium chloride 60~100 mmol / L, sodium azide 0.1~0.6 g / L, EDTA 12~26 mmol / L, 2-chloro-4-nitrobenzene-α-L-fucoside 18-72 mmol / L, the solvent is purified water. The kit provided by the invention produces specific hydrolyzed corresponding products, the reagents and products are non-toxic and harmless, the reaction is fast and sensitive, the detection is more accurate, the speed is fast, and there is no pollution; it can be directly detected and operated on various biochemical analyzers The method is simple and convenient, does not need to invest in expensive equipment, and saves detection costs.

Description

technical field [0001] The invention relates to the technical fields of medicine and biochemistry, in particular to a kit for measuring α-L-fucosidase. Background technique [0002] α-L-fucosidase (AFU) is a lysosomal acid hydrolase that widely exists in human tissue cells, blood and body fluids, and participates in the metabolism of glycoproteins, glycolipids and oligosaccharides. It is increased in the serum of patients with primary liver cancer and is one of the markers of primary liver cancer. [0003] 81.2% of patients with primary liver cancer have elevated serum AFU levels, and combined detection with AFP can increase the positive rate of primary liver cancer diagnosis to 93.1%. Dynamic observation is of great significance in judging the curative effect, prognosis and recurrence of liver cancer. Serum AFU can also be increased in metastatic liver cancer, lung cancer, breast cancer, ovarian cancer, and uterine cancer; it can also be slightly increased in liver cirrho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31
CPCG01N21/31
Inventor 蔡晓辉庄庆华吴铮徐运
Owner ANHUI IPROCOM BIOTECH CO LTD
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