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Preparation technology of porous extracellular matrix bracket

A technology of extracellular matrix and cells, applied in medical science, drug delivery, prosthesis, etc., can solve problems such as poor connectivity, uncontrollable structure, and too small pore size

Inactive Publication Date: 2016-10-12
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the currently prepared acellular matrix scaffold materials generally have the problems of uncontrollable structure, too small pore size and poor communication, which limits cell migration and the transmission of nutrients and metabolites, which in turn affects the remodeling and regeneration of repaired tissues and organs.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Example 1: Using PCL microsphere aggregates as a template to prepare a cell matrix scaffold with a controllable pore structure

[0012] Preparation of PCL microspheres: PCL microspheres were prepared by emulsification-solvent evaporation method. Weigh 10.0 grams of PCL with a molecular weight of 80,000, add it into 200 ml of dichloromethane, stir and dissolve at room temperature until clear, and prepare a PCL solution with a concentration fraction of 5% (m / v). The above PCL solution was added to 1800ml of 2% polyvinyl alcohol (PVA) solution, stirred at 1000rpm, volatilized at room temperature to remove dichloromethane, left to settle to collect the microspheres at the bottom, washed with distilled water 5 times to remove residual PVA, and collected The microsphere particles were freeze-dried to obtain PCL microspheres.

[0013] Preparation of PCL microsphere aggregates: place 0.5 g of PCL microspheres in a round hole with a diameter of 1.0 cm and a height of 0.8 cm on ...

Embodiment 2

[0016] Example 2: Using PLA microsphere aggregates as a template to prepare a cell matrix scaffold with a controllable pore structure

[0017] Preparation of PLA microspheres: Weigh 8.0 grams of PLA with a molecular weight of 200,000, add it to 200 ml of chloroform, stir and dissolve at room temperature until clear, and prepare a PLA solution with a concentration fraction of 4% (m / v). Above-mentioned PLA solution is added in the 3% polyvinyl alcohol (PVC) solution of 1800ml, stirs under 800rpm condition, volatilizes and removes chloroform at room temperature, stands and settles and collects microsphere, washes 5 times with distilled water to remove residual PVA, will collect The microsphere particles were freeze-dried to obtain PLA microspheres.

[0018] Preparation of PLA microsphere aggregates: place 0.4 g of PLA microspheres in a circular hole with a diameter of 1.0 cm and a height of 0.8 cm on a polytetrafluoroethylene plate, and heat at a constant temperature of 200 ° C f...

Embodiment 3

[0021] Example 3: Using PLGA microsphere aggregates as a template to prepare a cell matrix scaffold with a controllable pore structure

[0022] Preparation of PLGA microspheres: Weigh 10.0 grams of PLGA with a molecular weight of 20,000, add it to 100 ml of chloroform, stir and dissolve at room temperature until clear, and prepare a PLGA solution with a concentration fraction of 10% (m / v). Add the above PLGA solution to 1900ml of 1% PVA solution, stir at 1200rpm, remove chloroform by volatilization at room temperature, collect microspheres by static precipitation, wash with distilled water 5 times to remove residual PVA, and freeze-dry the collected microspheres .

[0023] Preparation of PLGA microsphere aggregates: 1.0 g of PLGA microspheres were placed in a round hole with a diameter of 2.0 cm and a height of 0.8 cm on a polytetrafluoroethylene plate, and heated at a constant temperature of 60 ° C for 40 minutes to make the microspheres Mutual bonding and melting, the PLGA ...

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Abstract

The invention discloses a preparation technology of a porous extracellular matrix bracket, aiming at solving the problems of an extracellular matrix bracket material that the pore diameter is small and cellularization is poor. Specifically, after a degradable polymer micro-sphere aggregate is planted into a subcutaneous tissue, muscle or an abdominal cavity of a host, a host cell is transferred into a micro-sphere aggregate pore to realize cellularization; after a cellularized micro-sphere aggregate bracket material is subjected to polymer elution and decellularization treatment in sequence, the extracellular matrix bracket can be obtained. The process can be used for controlling the size of the pore diameter of the bracket through controlling the size of the polymer micro-sphere; meanwhile, the connectivity of the bracket is controlled through controlling the splicing degree of the polymer micro-sphere. The porous extracellular matrix bracket prepared by the invention has a good application prospect in the fields of tissue engineering including stem cell transplantation, cartilage repairing, skin repairing and the like.

Description

technical field [0001] The invention relates to a preparation technology of a porous scaffold in the field of tissue engineering, in particular to a preparation technology of a highly connected extracellular matrix scaffold with controllable pore size. Background technique [0002] Scaffold materials are one of the three elements of tissue engineering. According to different sources, they are mainly divided into two categories: synthetic materials and natural materials. Among them, natural materials are derived from biological tissues or organs, and have better biocompatibility than synthetic materials, so they have always been a hot spot in tissue engineering research. Extracellular matrix scaffolds are a unique class of natural materials that are obtained by physically or chemically removing immunogenic components from organs or tissues. The structural components of the extracellular matrix are relatively complex, mainly including collagen, proteoglycan, elastin, fibronec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/56A61L27/50A61L27/36
CPCA61L27/3633A61L27/3687A61L27/3691A61L27/50A61L27/56A61L2400/08
Inventor 朱美峰孔德领王恺李雯
Owner NANKAI UNIV
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