Use of urea apolipoprotein C-II

A technology of C-II and apolipoprotein, applied in the application field of urine apolipoprotein C-II, can solve the problem of low level of plasma ApoC-II

Inactive Publication Date: 2016-10-05
张曼
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A defect in the intronic splice body site of the Apo C-II gene can lead to low levels of plasma Apo C-II

Method used

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  • Use of urea apolipoprotein C-II
  • Use of urea apolipoprotein C-II
  • Use of urea apolipoprotein C-II

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1 Urine Specimen Collection and Processing

[0017] Collect 30 cases of normal physical examination (Physical Examination Center of Beijing Shijitan Hospital Affiliated to Capital Medical University) and randomly clean midstream urine samples, centrifuge within 2 hours (1500rpm, 5min), and keep the supernatant. Store in a -80°C refrigerator after aliquoting.

Embodiment 2

[0018] Example 2 Magnetic Bead Purification and Isolation of Peptides from Urine Specimens

[0019] Take out the urine sample from the -80°C refrigerator, rethaw at 4°C, centrifuge (3000rpm, 10min), and take the supernatant for later use. Equilibrate Weak Cationic Magnetic Beads (MB-WCX) at room temperature and mix the magnetic bead suspension by hand. Add 10ul of MB-WCX and 10ul of magnetic bead binding buffer into the sample tube, pipette the sample gun up and down to mix well to avoid foaming. Add 5 ul of urine supernatant to the sample tube, mix well and let stand on the magnetic stand for 1 minute to separate the magnetic beads from the suspended liquid. Use a sampling gun to remove the suspended clear liquid, and the tip of the gun should avoid contact with the magnetic beads to avoid absorbing the magnetic beads. Add 100ul of magnetic bead washing buffer into the sample tube, mix well, and then place the sample tube on the magnetic stand for 1 minute, the magnetic ...

Embodiment 3

[0020] Example 3 Spot Targeting and Peptide Spectrum Generation of Urine Specimens

[0021] After calibrating the instrument with a standard, mix 1 μl of eluate with 10 μl of matrix (0.3% α-cyano-4-hydroxycinnamic acid, HCCA), and take 1 μl to spot on the Anchorchip (Autoflex MALDI TOF, Bruker-Dalton) target plate and dry at room temperature. The sample is ionized by nitrogen laser irradiation and then subjected to mass spectrometry analysis, collecting data in the range of 1000-10000 Da, and obtaining a mass spectrogram composed of protein peaks with different mass-to-charge ratios. For each MALDI crystallization point, a total of 400 laser irradiations (50 times for each crystallization point at 8 different positions) were irradiated, and the average value represented one sample, so as to obtain the peptide maps of all samples. ClinProTools2.1 analysis software was used to analyze the mass spectrograms of the normal control group, type 2 diabetes without complications an...

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Abstract

The invention provides a use of urea apolipoprotein C-II, Apo C-II and concretely relates to expression of the urea apolipoprotein C-II in urea and a use of the urea apolipoprotein C-II in urea content detection. A research proves that the urea apolipoprotein C-II has high expression so that disease diagnosis information can be obtained through the urea apolipoprotein C-II expression amount detection. The urea apolipoprotein C-II utilizes advantages of urea sample large-scale repeated sampling and utilizes a random urine sample to detect apolipoprotein C-II.

Description

technical field [0001] The invention relates to a new application of urine apolipoprotein C-II, in particular to the expression of apolipoprotein C-II in urine and its application in urine content detection. Background technique [0002] The Apo C-II gene is clustered with the ApoC-I and ApoE genes and is located in the q13 region of the long arm of chromosome 19. The Apo C-II gene is 3320bp long and contains 4 exons and 3 introns. ApoC-II is a single-chain polypeptide composed of 79 amino acid residues, without cysteine ​​and serine, and has two polymorphic types, the isoelectric points are 4.86 and 4.69, and the α-helix in its secondary structure is about 23%. Apo C-II is one of the structural proteins of CM, VLDL and HDL, accounting for 14%, 7%-10% and 1%-3% of their protein components respectively. Apo C-II is one of the most important subtypes in the Apo C family. It is a cofactor of LPL, plays an important role in lipid metabolism, and can activate lipoprotein lipas...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/535
CPCG01N33/48G01N33/53
Inventor 张曼付广真雷婷
Owner 张曼
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