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Combined tumor marker used for diagnosis breast cancer and kit thereof

A tumor marker and diagnostic reagent technology, applied in the field of combined tumor markers and their kits, can solve the problems of postoperative recurrence and metastasis affecting survival rate and mortality rate, etc.

Inactive Publication Date: 2016-10-05
SHANGHAI ADVANCED RES INST CHINESE ACADEMY OF SCI
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AI Technical Summary

Problems solved by technology

[0003] Surgical-based comprehensive treatment of breast cancer has achieved good results, but postoperative recurrence and metastasis are still key factors affecting survival and mortality

Method used

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  • Combined tumor marker used for diagnosis breast cancer and kit thereof
  • Combined tumor marker used for diagnosis breast cancer and kit thereof
  • Combined tumor marker used for diagnosis breast cancer and kit thereof

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0041] Example 1 Experiments on Cells and Breast Cancer Samples

[0042] (1) Experimental process

[0043]Materials and reagents: Fetal bovine serum was purchased from Gibco Company; penicillin-streptomycin for cell culture and TRIzol RNA extraction reagent were purchased from Invitrogen Company; RPMI-1640 and DMEM medium were purchased from Hyclone Company; KOD-plus, RealMasterMix (SYBR Green I) kits, reverse transcription kits were purchased from Dongyangfang Company; restriction endonucleases were purchased from Takara Company; T4DNA ligase, western and IP lysates were purchased from Beyontian Company; large quantities of plasmid extraction kits, plasmid small Quantitative extraction kit, gel recovery kit, and DNA product purification kit were purchased from Tiangen; Protein Inhibitor Cocktail and Phosphotase Inhibitor Cocktail were purchased from Roche Company; nitrocellulose membrane was purchased from Schleicher and Schuell Bioscience Company; goat polyclonal antibody AE...

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PUM

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Abstract

The invention discloses a combined tumor marker used for diagnosis the breast cancer and a kit thereof. The marker comprises asparagine endopeptidase and ubiquitin ligase TRAF6. The kit comprises a diagnostic reagent for detecting the expression condition or the expression amount of asparagine endopeptidase or a coding gene thereof, and a diagnostic reagent for detecting the expression condition or the expression amount of ubiquitin ligase TRAF6 or a coding gene thereof. The AEP and TRAF6 can be combined as the marker for combined detection of the breast cancer; the commercial kit is developed for diagnosis of the early, middle and late periods of the breast cancer; sodium cotton ketone taurate can be used as a candidate medicine for inhibition of breast cancer metastasis by down-regulating AEP and protein activated by AEP; so the marker and the kit provided by the invention lay a foundation for development of breast cancer medicines.

Description

technical field [0001] The invention relates to a tumor marker, in particular to a combined tumor marker for breast cancer diagnosis and a kit thereof. Background technique [0002] According to WHO statistics, there are more than 1 million new cases of breast cancer in the world every year, and about 500,000 people die of breast cancer every year in the world. BF. Unraveling the role of proteases in cancer. Clinica Chimica Acta 2000; 291(2):113-135). In developed countries such as Western Europe and North America, the incidence of breast cancer ranks first among female malignant tumors. The latest "Report on Breast Cancer Prevention and Control in China" released by the Health Education Institute of the Chinese Center for Disease Control and Prevention shows that breast cancer has become the most common malignant tumor that threatens women's health, and China is one of the countries with the fastest growing incidence of breast cancer. Statistics released by the China Anti-...

Claims

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Application Information

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IPC IPC(8): G01N33/574
Inventor 郭方王雪枫严向明
Owner SHANGHAI ADVANCED RES INST CHINESE ACADEMY OF SCI
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