11-dehydro-thromboxane b2 assay kit and its application
A kit and thromboxane technology, applied in the field of 11-dehydro-thromboxane B2 assay kits, can solve the problems of consuming a lot of manpower, material resources and time, it is difficult to automatically detect batches, and the precision is low, and achieve accuracy High, high sensitivity, good precision effect
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Embodiment 1
[0033] Example 1: Preparation of 11-dehydro-thromboxane B2 assay kit
[0034] 1. Preparation of reagent R1:
[0035] 0.015 mg of the 11dhTxB2 monoclonal antibody was weighed and dissolved in 100 ml of 10 mmol / L Tris buffer to make it completely dissolved, and filtered through a 0.22 μm membrane to prepare 0.015% (W / V) reagent R1.
[0036] 2. Preparation of reagent R2:
[0037] Using 30nm hydroxylated polystyrene nano latex particles, add EADC:Sulfo-NHS:Nanolatex particles to MES buffer with pH=6.0, the mass ratio is 1:0.5:0.1, ultrasonic for 2-3 seconds, and mix well , activated on a rotary shaker for 2 hours at room temperature, centrifuged at 17,000 rpm for 30 minutes, washed 3 times with MES buffer with pH=6.0, and then suspended the microspheres in HEPES buffer with pH=8.0 to make a microparticle suspension, and measure the particles. diameter and CV value.
[0038] To the 30nm particle suspension, add 11dhTxB2 protein with a mass of 1 / 3 the weight of the nanolatex part...
Embodiment 2
[0043] Example 2: Preparation of 11-dehydro-thromboxane B2 assay kit
[0044] The difference from Example 1 is that 2% (W / V) reagent R1 and 2% (W / V) reagent R2 were prepared, wherein 400 nm hydroxylated acrylate nanolatex particles were used in the preparation of reagent R2.
Embodiment 3
[0045] Example 3: Preparation of 11-dehydro-thromboxane B2 assay kit
[0046] The difference from Example 1 is that 1% (W / V) reagent R1 and 1% (W / V) reagent R2 are prepared, wherein 200 nm hydroxylated divinylbenzene nanolatex particles are used in the preparation of reagent R2 .
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