Nerve cells protection method

A nerve cell protection and nerve cell technology, applied in the field of biomedical engineering, can solve problems such as unreported, and achieve the effects of low price, wide clinical application prospects, and simple operation.

Active Publication Date: 2016-09-28
北京北卓医疗科技发展有限责任公司
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Problems solved by technology

There is no report about the protective effect of plasma on nerve cells

Method used

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Embodiment 1

[0022] Embodiment 1: Nerve cell protection experiment of plasma

[0023] 1. Experimental materials

[0024] 1. SH-SY5Y cells were purchased from the Cell Resource Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences.

[0025] 2. 1640 medium and FBS (Fetal Bovine Serum) were purchased from Gibco.

[0026] 3. Plasma device: Provided by Beijing Institute of Technology, it is a coaxial coplanar DBD discharge device. The inner diameter of the dielectric tube is 1mm, the outer diameter is 2mm, and the two electrodes are wound by metal aluminum foil with a width of 3mm. The left electrode is a power electrode , the driving power is an alternating current signal with a frequency of f=30kHz, the electrode on the right is a ground electrode, and high-purity helium flows in from the left end of the dielectric tube at a fixed flow rate.

[0027] 4. CCK-8 cell proliferation and activity detection kit: purchased from Tongren Institute of Chemistry (CK04).

[0...

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PUM

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Abstract

The invention discloses a nerve cells protection method, which belongs to the field of biomedical engineering. The nerve cells protection method is characterized in that the nerve cells culture by plasma, the research finds that small dosage is employed for processing cells with short time, and cells can be effectively protected. The method firstly provides a plasma cells protection conception, and firstly proves that the low dosage plasma treatment can effectively protect H2O2-damaged nerve cells, the method provides novel thinking and the method for preventing and treating nerve cell damage restoration and prevention and treatment of degenerative diseases, the cost is lower, the operation is simpler, and the method has the meaning for developing animal in-vivo study and clinic researches, and has wide clinic application.

Description

technical field [0001] The invention relates to a nerve cell protection method, which belongs to the field of biomedical engineering. Background technique [0002] Cerebrovascular disease is a common and frequently-occurring disease that endangers human life and health. It is currently the number one fatal and disabling disease in my country. Among the survivors, 50%-70% of patients are left with severe disabilities such as paralysis and aphasia, which brings great harm to society and families. heavy burden. More and more data prove that the irreversible degeneration and loss of neurons in specific parts of the brain is an important cause of neurological diseases. There are many hypotheses about its inducing mechanism, such as neurotransmitter imbalance theory, mitochondrial dysfunction theory, excitotoxicity theory, abnormal protein accumulation theory, gene mutation, inflammatory process, immune abnormality and cell apoptosis theory. In these hypotheses, oxidative stress ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/079A61K33/00A61P25/00A61P9/10
CPCA61K33/00C12N5/0618
Inventor 闫旭欧阳吉庭袁芳乔雅隽
Owner 北京北卓医疗科技发展有限责任公司
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