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Application of salicylhydroxamic acid to detection of hypochlorite

A technology of salicyl hydroxamic acid and hypochlorite, which is applied in measuring devices, instruments, fluorescence/phosphorescence, etc., can solve the problems of high cost and complicated process, achieve high sensitivity and selectivity, and the detection process is simple, The effect of accurate test results

Inactive Publication Date: 2016-09-07
SHANXI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, most detection methods have problems such as complex process, detection reagents need organic synthesis process, and high cost

Method used

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  • Application of salicylhydroxamic acid to detection of hypochlorite
  • Application of salicylhydroxamic acid to detection of hypochlorite
  • Application of salicylhydroxamic acid to detection of hypochlorite

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Prepare 2mM salicylhydroxamic acid solution with methanol; add 2mL of methanol solution and 2.5μL of salicylhydroxamic acid methanol solution to a clean fluorescent cuvette, take NaClO solution, and gradually use micro-injection Add the sample into the cuvette, and detect it on the fluorescence spectrophotometer while adding the sample. With the addition of NaClO, the fluorescence intensity at 415nm gradually increases. Fluorescence emission map see figure 1 .

Embodiment 2

[0019] Prepare 2mM NaClO solution with methanol; add 2mL methanol solution and 2.5μL NaClO methanol solution to 12 fluorescent cuvettes, then add 1 molar equivalent of NaClO and 50 molar equivalents of other analytes (ClO - ,H 2 o 2 , 1 o 2 ,·O 2 - , ROO , NO , OH), detected on the fluorescence spectrophotometer, draw the histogram of the 415nm fluorescence intensity corresponding to different analytes, and obtain the fluorescence emission diagram, see figure 2 (NaClO makes the fluorescence intensity of salicyl hydroxamic acid change from 24 to about 787, and other analytes basically do not cause the change of the fluorescence intensity of curcumin).

[0020] It has been proved by experiments that other analytes do not interfere with the determination of NaClO by the system.

Embodiment 3

[0022] Prepare 2mM NaClO solution with distilled water, add 2mL of methanol solution and 2.5μL of salicylhydroxamic acid methanol solution to the fluorescence cuvette, gradually add the volume of NaClO solution to 0.17, 0.35, 0.50, 0.60, 0.70, 0.95, 1.0, 1.25, 1.36, 1.54, 1.64uL, and the corresponding fluorescence intensity F at 415nm measured on the fluorescence spectrometer is 65, 156, 233, 284, 325, 457, 496, 589, 650, 736, 787, to The concentration of NaClO is the abscissa, and the relative fluorescence intensity F-F 0 Plot the graph for the ordinate, F 0 ﹦24, get the working curve of NaClO concentration; the linear regression equation is: F-F 0 -=489.232c-39.2948, the unit of c is 10 -6 mol / L; see image 3 .

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Abstract

The invention provides application of salicylhydroxamic acid to detection of hypochlorite and specifically discloses a fluorescence detection method for hypochlorite. The detection method uses salicylhydroxamic acid for quantitative detection of the content of hypochloric acid in a HEPES solution with a pH value of 7.4. The detection method shows high sensitivity and selectivity to hypochlorite; a detection reagent is cheap; detection process is easy, sensitive and rapid; and detection results are accurate.

Description

technical field [0001] The invention relates to hypochlorous acid detection and analysis technology, in particular to the application of salicylhydroxamic acid in the detection of hypochlorite. Background technique [0002] Sodium hypochlorite concentrations range from 10 -5 to 10 -2 mol / L is widely used in bleaches and disinfectants. However, high concentrations of sodium hypochlorite are extremely harmful to human and animal health. On the other hand, hypochlorite ion is one of the biologically important reactive oxygen species (ROS), which plays an important role in the immune system. endogenous CLO - It is the basic substance of life and has antibacterial properties. However, abnormalities in hypochlorite can lead to tissue damage and diseases, such as, liver ischemia-reperfusion injury, atherosclerosis, lung injury, rheumatoid arthritis, cardiovascular disease, neuronal degeneration, arthritis, and cancer. Therefore, the detection of hypochlorous acid in environm...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6428
Inventor 阴彩霞霍方俊
Owner SHANXI UNIV
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