An industrial ultra-high pressure cell crushing method and a cell crushing machine

Abstract

The invention discloses an industrial ultrahigh-pressure cell disruption method. According to the method, cells to be disrupted are subjected to volume two-stage expansion; then, the cells are disrupted by using a void effect and a shearing effect; the obviously expanded volume can enhance the disruption effects of the void effect and the shearing effect; the disruption effect better than the direct void effect and shearing effect cell disruption can be achieved, so that the cell tissues in biological medicine raw materials can be crushed; more than 97 percent of cell tissues can reach the value being less than 100nm; particles of target objects are uniform; the further extraction and purification are convenient; a great number of chemical reagents can be saved; the industrial pollution is reduced; and the utilization rate of medicine raw materials is greatly improved. The invention also discloses an industrial ultrahigh-pressure cell disruption machine, which comprises a support frame, a two-way hydraulic oil cylinder, an ultrahigh-pressure cavity, a recovery cavity, a check valve, at least one discharging valve, a feeding hopper, a pneumatic oil pump, an oil tank and a two-position four-way electromagnetic reversing valve.

Description

Technical field [0001] The invention relates to the technical field of biomedicine, in particular to an industrial ultra-high pressure cell crushing method and a cell crusher. Background technique [0002] With the development of science and technology, it has been discovered that the active ingredients in the raw materials of Chinese medicine and biomedicine that can inhibit or treat diseases are generally present in the cells of animals and plants, and the content is very low. For example, paclitaxel, which has a good effect in the treatment of breast cancer and ovarian cancer, contains about 0.015% in high-quality yew; the content of anthocyanin mixture with strong antioxidant effect in blueberries is about 0.16%. The above-mentioned paclitaxel mainly exists in the dry branches, roots and bark of Taxus chinensis. Similar to this plant, the effective ingredients of many Chinese herbal medicines will increase with the growth time of the plant, and the longer the growth time, the...

AI Technical Summary

Problems solved by technology

It often causes social problems such as a large amount of waste of raw materials, increase of by-products, and environmental pollution caused by a large amount of chemical reagents, and is not suitable for large-scale industrial use.

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