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Preparation method of solid-liquid bidirectional acidified glycolysis feed

A feed and fermentation technology, applied in the field of enzyme preparations, microorganisms, and feed, can solve the problems of complex process, large investment in fermentation equipment, and expensive raw materials.

Inactive Publication Date: 2016-09-07
FLSUGARPEPTIDE BIOLOGY ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] 2. The raw materials of the two patent mediums are all starch-containing products, it is difficult to use fibers and waste residues, and the raw materials are expensive
[0014] 3. The two patents are for the production of citric acid by liquid fermentation, which requires a large amount of steam, complex processes, large investment in fermentation equipment, heavy pollution, and high water and power consumption.
[0015] 4. Neither of the two patents involves the process of direct crystallization of citric acid in the state of pigment and ion and the technology of solid-state fermentation material adsorption of mother liquor
[0016] 5. Neither of the two patents has the technology of directly mixing citric acid fermentation liquid, lactic acid fermentation liquid and other organic acid fermentation liquids with the wet base of fermented dregs medium to prepare acidified and fermented feed
[0017] 6. The final mixing process of the two patents does not involve the addition of Chinese herbal medicine and vitamin technology

Method used

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  • Preparation method of solid-liquid bidirectional acidified glycolysis feed
  • Preparation method of solid-liquid bidirectional acidified glycolysis feed
  • Preparation method of solid-liquid bidirectional acidified glycolysis feed

Examples

Experimental program
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Effect test

Embodiment 1

[0072] The preparation of a milk lemon compound solid-liquid two-way acidification fermented feed comprises the following steps:

[0073] Crush 100kg of broken rice, add 400kg of water to adjust the slurry, add liquefying enzyme and glucoamylase for enzymatic hydrolysis, and obtain 500kg of saccharified liquid with slag. Through solid-liquid separation, 18kg of sugar residue wet base and 482kg of residue-free saccharification liquid were obtained.

[0074] In 482kg of slag-free saccharification liquid, heat up, sterilize and cool down, insert 20kg of lactic acid bacteria seed solution that produces lactic acid, and carry out under the basic constant conditions of continuous flow of calcium hydroxide solution, temperature 42-45°C, and fermentation pH: 7. Anaerobic fermentation was carried out for 48-60 hours to obtain 482 kg of crude lactic acid fermentation liquid. Add 60kg of 20% dilute sulfuric acid solution to 482kg of lactic acid fermentation broth, acidify to generate ca...

Embodiment 2

[0080] The preparation of a milk lemon compound solid-liquid two-way acidification fermented feed comprises the following steps:

[0081] Grind 100kg of corn, add 300kg of water to adjust the slurry, add liquefaction enzyme and glucoamylase for enzymatic hydrolysis, and obtain 400kg of saccharification liquid with slag. Through solid-liquid separation, 70-75kg of sugar residue wet base and 325-330kg of residue-free saccharification liquid are obtained.

[0082] In 325-330kg slag-free saccharification liquid, heat up, sterilize and cool down, insert 15kg of lactic acid-producing lactic acid bacteria seed solution, add calcium hydroxide solution in continuous flow, temperature 42-45°C, fermentation pH: 7 under basically constant conditions , carry out anaerobic fermentation for 48-60 hours, and obtain 340-345kg of crude lactic acid fermentation liquid. Add 40kg of 20% dilute sulfuric acid solution to 340-345kg of lactic acid fermentation broth to acidify to generate calcium sul...

Embodiment 3

[0088] The preparation of a milk lemon compound solid-liquid two-way acidification fermented feed comprises the following steps:

[0089] De-iron the molasses. Weigh 100kg of molasses that has been freed from iron and iron, and add 250kg of water to adjust the slurry to obtain 350kg of slag-free syrup.

[0090] In 350kg of slag-free sugar solution, heat up, sterilize and cool down, insert 20kg of lactic acid bacteria seed solution that produces lactic acid, and carry out under the basic constant conditions of continuous flow of calcium hydroxide solution, temperature 42-45°C, and fermentation pH: 7. Anaerobic fermentation was performed for 48-60 hours to obtain 368-369 kg of crude lactic acid fermentation liquid. Add 30kg of 20% dilute sulfuric acid solution to 368-369kg of lactic acid fermentation broth, acidify to generate calcium sulfate and lactic acid. Through solid-liquid separation, 25kg of lactic acid residue wet base and calcium sulfate wet base, and 373-374kg of la...

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Abstract

The invention relates to a preparation method of a solid-liquid bidirectional acidified glycolysis feed. The preparation method comprises the following steps of separately inoculating organic acid strains into a liquid culture medium and a solid culture medium, and performing separate fermentation to produce liquid fermentation source organic acid and solid fermentation source organic acid; and mixing the liquid fermentation source organic acid and the solid fermentation source organic acid with one or more of inorganic acid for feeds, organic acid salt, saccharide, grease, Chinese herbal medicines and vitamins, and performing baking so as to obtain the solid-liquid bidirectional acidified zymohydrolysis feed. The feed prepared by the method disclosed by the invention contains nutrients including lactic acid, citric acid, other organic acid, protein, crude fibers and the like, so that the feed has the characteristics of resisting diarrhoea, being good in palatability, being easy to digest and the like.

Description

technical field [0001] The invention belongs to the technical field of feed, enzyme preparations and microorganisms, and in particular relates to a preparation method of a solid-liquid two-way acidification fermented feed. Background technique [0002] In recent years, food safety and environmental pollution have been widely concerned by governments at all levels in various countries, which are related to the health and life safety of the people. The safety of animal products requires that the feed has no drug residues, and the environmental protection requires that the feed has low pollution. In the past, we talked about "effective and safe" feed, but now the Ministry of Agriculture has repeatedly emphasized "safe, effective and low pollution". As we all know, since the advent of antibiotics, their use in livestock and poultry to prevent diseases and stimulate growth has indeed received good results. However, with the passage of time, the defects of antibiotics have been f...

Claims

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Application Information

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IPC IPC(8): A23K10/12A23K20/24A23K20/163A23K10/30A23K10/33A23K10/37A23K20/105A23K20/142A23K20/147A23K10/38A23K10/28A23K10/20A23K10/35A23K20/158
CPCY02P60/87
Inventor 程勐万里刘萱仪孟龙薛芹程茂基
Owner FLSUGARPEPTIDE BIOLOGY ENG
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