5‑lipoxygenase pathway protein joint prediction kit for prognosis of patients with esophageal cancer
A technology of lipoxygenase and kit, which is applied in the field of 5-lipoxygenase pathway protein joint prediction kit for the prognosis of patients with esophageal cancer, can solve the problems of little research
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[0018] We collected 297 surgical specimens of esophageal cancer patients from 2007 to 2011. The following is a further detailed description in conjunction with the embodiments of the drawings.
[0019] The steps of the immunohistochemical hypersensitivity two-step method are as follows.
[0020] After the specimen is serially sectioned, deparaffinized with xylene, and hydrated with gradient alcohol, the expression of the target protein is detected by the hypersensitive two-step method. The specific method is as follows.
[0021] (1) The slices are restored with 0.01M citrate repair solution, 3% H 2 O 2 After inactivating endogenous peroxidase and blocking with goat serum, they were incubated with 5-LO, FLAP, and LTA4H antibodies at 4°C overnight.
[0022] (2) The sections were incubated with Polymer enhancer and Polymer polymer and then developed by DAB.
[0023] (3) The sections were counter-stained with hematoxylin, dehydrated with gradient alcohol, and mounted with transparent xylen...
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