Method using ASE method to extract linarin in buddleja officinalis

A technology of cypress florets and cylindrosides, which is applied in the field of laboratory testing, can solve the problems of high requirements on operators' operating skills, complicated pharmacopoeia operation process, etc., and achieves the effects of short extraction time, simple operation and high extraction precision.

Inactive Publication Date: 2016-08-24
WUZHOU INST FOR FOOD & DRUG CONTROL
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  • Abstract
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Problems solved by technology

[0004] The operation process of the method in the Pharmacopoeia is complicated and requires high skills of the operator

Method used

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  • Method using ASE method to extract linarin in buddleja officinalis
  • Method using ASE method to extract linarin in buddleja officinalis
  • Method using ASE method to extract linarin in buddleja officinalis

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Embodiment 1

[0024] Pulverize the Mimeng flower through a pulverizer, pass through a No. 3 sieve, weigh about 0.5g, and mix it with 0.5g of diatomaceous earth. Add an appropriate amount of diatomaceous earth to the 10ml extraction pool, shake gently to make it on the same level as the pool mouth, and tighten the upper cover of the extraction pool. After the extraction, transfer the extract to a 50ml volumetric flask, dilute to the mark with methanol, centrifuge at 15000r / min for 5min, take the supernatant, and enter it into LC for determination.

[0025] The extraction conditions (see table 1) are:

[0026] Table 1

[0027]

[0028] Analysis method is LC liquid chromatography, liquid chromatography analysis conditions:

[0029] a) Instrument: Thermo double ternary liquid phase (U-3000);

[0030] b) Chromatographic column: Thermo Syncronis C18 3*100mm 3um, that is, a carbon-18 chromatographic column with a diameter of 3mm, a length of 100mm, and a particle size of 3 microns;

[0031...

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Abstract

The invention discloses a method using an ASE method to extract linarin in buddleja officinalis. The method includes the steps of firstly, crushing the buddleja officinalis, and sieving; secondly, weighing 0.5 part of buddleja officinalis powder, and evenly mixing with 0.5 part of diatomite for standby; thirdly, adding an appropriate amount of diatomite into an extraction tank with a preset filter membrane until the diatomite reaches the opening of the extraction tank, and covering the extraction tank; fourthly, performing ester removing on the mixture in the extraction tank; fifthly, extracting the mixture in the extraction tank after the fourth step to obtain extract. The method is simple to operate and high in detection precision.

Description

technical field [0001] The invention relates to the field of laboratory detection, in particular to an ASE method for extracting the glucoside of the flower of Budisma japonica. Background technique [0002] In 2015, the Pharmacopoeia recorded the method of extracting fenugreek from Budisma japonica: Take about 0.5g of the coarse powder of this product, weigh it accurately, put it in a Soxhlet extractor, add 100ml of petroleum ether (60-90°C), and heat to reflux for 2 hours, discard the petroleum ether, evaporate the dregs, add 100ml of methanol and continue to heat and reflux for 4 hours, put the extract in an evaporating dish, concentrate to an appropriate amount, and transfer it to a 50ml measuring bottle. Incorporate into the same measuring bottle. Add methanol to the mark, shake well, filter, and take the continued filtrate, that is, too. [0003] The parameters of its liquid chromatography are: octadecylsilane bonded silica gel as filler; methanol-0.1% glacial acetic ...

Claims

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Application Information

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IPC IPC(8): G01N30/06
CPCG01N30/06G01N2030/062
Inventor 陈学松王丽丽韦涛梁美艳黄新惠
Owner WUZHOU INST FOR FOOD & DRUG CONTROL
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