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Primer and method for fast typing campylobacter coli

A rapid technology for Campylobacter colon, applied in biochemical equipment and methods, microbial determination/examination, DNA/RNA fragments, etc. High accuracy and specificity

Active Publication Date: 2016-08-24
GUANGDONG PHARMA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, there is no report on the genotyping of Campylobacter coli using AILP technology.

Method used

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  • Primer and method for fast typing campylobacter coli
  • Primer and method for fast typing campylobacter coli
  • Primer and method for fast typing campylobacter coli

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1, primer

[0024] The primer provided by the invention is used for rapid typing of Campylobacter coli.

[0025] Table 1 Primers provided by the present invention

[0026]

[0027]

Embodiment 2

[0028] Example 2, the length of each target gene interval segment of Campylobacter coli

[0029] The length of the murD target intergenic fragment provided by the present invention is 117bp, the length of the tpx target intergenic fragment is 465bp, the length of the proB target intergenic fragment is 760bp, the length of the ybjZ target intergenic fragment is 1155bp, and the length of the msrA target intergenic fragment is The length of the target intergenic fragment of purB is 1306bp, the length of the target intergenic fragment of purB is 1638bp, the length of the target intergenic fragment of moeA-3 is 1910bp, the length of the target intergenic fragment of mpg is 2210bp, the length of the target intergenic fragment of rplW is 2777bp, and the target intergenic fragment of proP The length of the fragment is 3251bp, the length of the typA target intergenic fragment is 212bp, the length of the mutY target intergenic fragment is 263bp, the length of the glnP target intergenic f...

Embodiment 3

[0032] Embodiment 3, a kind of method for rapid typing of Campylobacter coli

[0033] 1. Extraction of Genomic DNA of Campylobacter coli:

[0034] Take the 44 strains of Campylobacter coli to be tested, respectively densely streak on the blood agar plate medium, place in 5% O 2 , 10% CO 2 and 85%N 2 Under microaerophilic conditions, after 24-48 hours of enrichment culture at 37°C, scrape all the colonies on the medium to 2mlddH 2 In O, vortex oscillating and mixing, then centrifuge at 10000rpm for 2min, discard the supernatant, and operate according to the bacterial genomic DNA small-scale purification kit of Takara Company, respectively extract 44 strains of Campylobacter coli genomic DNA, the extracted DNA The concentration is 10-200ng / μl. The obtained genomic DNA was stored at -20°C before testing.

[0035] 2. PCR amplification of different gene interval segments of the strain to be tested:

[0036] With 15 pairs of Campylobacter coli primers for Campylobacter coli ge...

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PUM

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Abstract

The invention belongs to the field of microorganism molecular typing and particularly relates to a primer and a method for fast typing campylobacter coli. The primer includes 15 pairs of campylobacter coli primers. The primer is good in typing capacity, high in distinguishing index and capable of accurately typing the campylobacter coli. In addition, the method for fast typing the campylobacter coli is simple and fast to operate, good in stability and repeatability, capable of effectively assisting the clinical diagnosis of diseases infected by the campylobacter coli and ideal.

Description

technical field [0001] The invention belongs to the field of microbial molecular typing, and in particular relates to a primer and a method for rapid typing of Campylobacter coli. Background technique [0002] Campylobacter belongs to Gram-negative short bacilli, and is an important class of food-borne zoonotic pathogens, which are widely distributed in nature and in the intestines of livestock and poultry. Since Dekeyser and Butzler isolated Campylobacter from the feces of patients with acute enteritis in 1973 and determined its pathogenicity for the first time, at least 16 different types of Campylobacter have been found, including Campylobacter jejuni and Campylobacter coli. coli) are the two most common strains of Campylobacter infection, accounting for more than 99%, of which 80-90% are Campylobacter jejuni, 5-10% are Campylobacter coli, and other common Campylobacter include Campylobacter hiou Campylobacter lari, Campylobacter fetus and Campylobacter upsaliensis, etc....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12Q1/04C12N15/11
CPCC12Q1/689
Inventor 毕水莲罗永文
Owner GUANGDONG PHARMA UNIV
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