Streptococcus thermophilus and application thereof
A technology of Streptococcus thermophilus and strains, applied in the field of microbiology, can solve the problems of imperfect preparation of Streptococcus thermophilus starter, poor strain viability, low exopolysaccharide production, etc., to achieve large-scale industrial application and improve quality Structural characteristics, the effect of simple process
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[0023] The preparation method of the culture medium used in the embodiment is as follows:
[0024] Skim milk culture solution: skim milk powder 120g / L, sterilized at 115°C for 15min.
[0025] Skim milk medium: skim milk powder 120g / L, bacterial agar powder 20g / L, sterilized at 115°C for 15min.
Embodiment 1
[0026] Example 1. Screening and Identification of Streptococcus thermophilus AR-333 Strain
[0027] 1. Sample dilution preparation and strain isolation
[0028] Add 1 g of naturally fermented dairy products to 15 mL of sterile water, and shake vigorously for 10 min. Use concentration gradient dilution method to dilute, take 100uL of the diluted solution and spread it on the skim milk agar medium plate, culture it anaerobically at 35~42℃ for 40~56h, select the strain with large colony, fast growth and high viscosity of filament, and further draw Line separation and purification. In this embodiment, the preferred culture temperature is 42° C., and the preferred anaerobic culture time is 48 h.
[0029] 2. Primary screening of strains
[0030] Inoculate the isolated strain with high thread-picking viscosity in 1.1 into the skim milk liquid medium, and culture it statically at 35-42°C for 8-12 hours to prepare a seed liquid; then inoculate the seed liquid with an inoculum volume...
Embodiment 2
[0049] Embodiment 2. Using Streptococcus thermophilus AR-333 strain as starter to prepare fermented milk and cheese
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