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Method for authenticating potato resource cold tolerance and application thereof

An identification method, a potato technology, is applied in the field of identification and analysis of crop stress resistance, which can solve the problem of few molecular markers, and achieve the effects of low cost, low requirements for analysis conditions, and easy separation and extraction

Active Publication Date: 2016-08-17
HUNAN AGRICULTURAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, few molecular markers are currently available

Method used

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  • Method for authenticating potato resource cold tolerance and application thereof
  • Method for authenticating potato resource cold tolerance and application thereof
  • Method for authenticating potato resource cold tolerance and application thereof

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Experimental program
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Embodiment

[0047] Materials to be identified: Feiwu Ruita, BS226, BS214, Yan2, 13*10, 41*8, Zhongshu No. 3 (verified as a common potato cultivar with weak cold resistance by the Institute of Vegetables and Flowers of Chinese Agricultural Sciences), Xingjia Number two.

[0048] Control: GS393 (strong cold-tolerant wild potato species provided by The U.S. National Plant GermplasmSystem NPGS, collected and preserved by Hunan Potato Engineering Technology Center, and distributed), S4, Q304, AV9; substrate potted plants , Substrate using commercial vegetable cultivation soil.

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Abstract

The invention discloses a method for authenticating potato resource cold tolerance and application thereof. The method comprises the following steps: 1) respectively sampling mature leaves of potatoes to be authenticated and mature leaves of cold tolerant potatoes as control; 2) keeping the leaves in a low-temperature environment with the initial temperature set as 0 DEG C for 20-40 minutes, and sampling so as to obtain a sample 1; 3) reducing the temperature to -1 DEG C from 0 DEG C, keeping the temperature for 50-70 minutes, and sampling so as to obtain a sample 2; 4) reducing the temperature to minus 2 DEG C from minus 1 DEG C, keeping the temperature for 50-70 minutes, and sampling so as to obtain a sample 3; 5) measuring the content of soluble sugar of the samples 1, 2 and 3; 6) performing clustering analysis on data by using DPS analysis software, and drawing diagrams; 8) obtaining the cold tolerance of the potatoes to be authenticated according to the diagram results. The method is rapid in material treatment, simple in item analysis, large in quantity of materials treated in a single batch, capable of implementing primary cold tolerance grading on a great amount of materials within a short time, high in detection efficiency and low in detection cost.

Description

technical field [0001] The invention belongs to the technical field of identification and analysis of crop stress resistance, and relates to a gradient low-temperature treatment of isolated potato leaves, analyzing the soluble sugar content of the leaves under different treatment conditions, using cold-resistant materials as a control, and systematically clustering analysis materials and contrast European-style An analytical method for analyzing potato cold tolerance by distance. Background technique [0002] There are more than 300 million mu of idle winter fields in rice production areas in southern my country. Planting potatoes in idle winter fields is a new and effective measure to improve my country's food security level. The planting area of ​​winter potatoes in my country has reached more than 30 million mu. Because potatoes prefer warm temperatures and are afraid of cold, winter potato seedlings are mostly in low temperature, rainy weather and frequent frosts, which...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/00G01N27/06
CPCG01N27/06G01N33/0098
Inventor 秦玉芝熊兴耀刘明月胡新喜宋勇何长征潘飞谢洁丁旭
Owner HUNAN AGRICULTURAL UNIV
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