Microbe concentration detection method based on immunomagnetic separation and biological catalysis

Abstract

The invention discloses a microbe concentration detection method based on immunomagnetic separation and biological catalysis. The method comprises the following steps: separating and enriching target microbes through a first bio-recognition element modified immunomagnetic element to improve the concentration and the purity of the target microbes; simultaneously modifying a nano-element with a second bio-recognition element and a predetermined enzyme, introducing an enzyme for catalyzing a biological reaction to a detection system in a sandwich manner, and changing the pH value of a catalysis solution by using the predetermined enzyme catalyzed biological reaction; and adding a pH indicator, and determining the content of the target microbes in the sample to be detected according to the color change of the pH indicator. The method integrates the purification and enrichment characteristics of immunomagnetic separation, the catalysis amplification characteristic of biological catalysis and the simple determination characteristic of the pH indicator, and also has the advantages of effective improvement of the detection sensitivity, shortening of the detection time, simplification of the detection process, and reduction of the detection cost.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a method for detecting the concentration of microorganisms based on immunomagnetic separation and biocatalysis. Background technique [0002] At present, my country is facing a severe food safety situation, and food safety incidents occur frequently. According to statistics from the National Center for Disease Control and Prevention, more than 50% of food poisoning incidents in my country from 2005 to 2014 were caused by microorganisms. The key to the prevention and control of food safety lies in the early detection, early warning and early treatment of pathogenic microorganisms. It is of great significance to establish sensitive and rapid detection methods for pathogenic microorganisms. Although domestic and foreign researchers have carried out a lot of research on improving the sensitivity of microbial detection and reducing the detection time, it is still a huge challenge ...

AI Technical Summary

Problems solved by technology

Although domestic and foreign researchers have carried out a lot of research on improving the sensitivity of microbial detection and reducing the detection time, it is still a huge challenge to realize the rapid detection of pathogenic microorganisms in food, mainly because the number of pathogenic microorganisms in contaminated food Usually less, coupled with the complex food matrix, it is difficult for conventional analysis methods to detect pathogenic microorganisms with high sensitivity and specificity

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