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Preparation method of antimicrobial bacterial cellulose material

A technology of bacterial cellulose and organosilicon quaternary ammonium salt, which is applied in the direction of medical preparations of non-active ingredients, pharmaceutical formulations, medical science, etc., can solve the problems of limited load capacity, easy shedding, bioaccumulation, and toxic and side effects, and achieve Mild and simple reaction conditions, good mechanical strength and high reaction efficiency

Inactive Publication Date: 2016-08-17
DONGHUA UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the one hand, the loading amount of nano-silver is very limited, the antibacterial effect of low-dose loading is not obvious, and high-dose loading will produce obvious bioaccumulation and toxic side effects; on the other hand, the binding stability of nano-silver in bacterial cellulose is poor, and it is easy to fall off. , not long-lasting

Method used

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  • Preparation method of antimicrobial bacterial cellulose material
  • Preparation method of antimicrobial bacterial cellulose material
  • Preparation method of antimicrobial bacterial cellulose material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The amino-functionalized bacterial cellulose material was prepared according to the following steps:

[0040](1) Add 60g of N,N-dimethyl-N-[3-(trimethoxysilyl)propyl]octadecylammonium chloride to 40g of methanol solution to prepare 60% mass ratio of N,N-dimethoxy Methyl-N-[3-(trimethoxysilyl)propyl]octadecylammonium chloride solution;

[0041] (2) Add 10 mL of deionized water to a 25 mL beaker, then add 10 pieces of purified bacterial cellulose wet membranes with a diameter of 1.5 cm, place the beaker in a water bath magnetic stirrer, and the reaction conditions are 25 ° C and 20 rpm;

[0042] (3) Add 0.23mL of prepared N,N-dimethyl-N-[3-(trimethoxysilyl)propyl]octadecylammonium chloride solution to the above system, the final concentration is 3.1×10 -3 mmol / L;

[0043] (4) Add glacial acetic acid dropwise to adjust the pH to 4.0, and continue stirring for 48 hours at 25°C and 20 rpm;

[0044] (5) Collecting the reacted bacterial cellulose membrane, washing with deio...

Embodiment 2

[0054] The amino-functionalized bacterial cellulose material was prepared according to the following steps:

[0055] (1) Add 10 mL of deionized water to a 25 mL beaker, then add 10 pieces of purified bacterial cellulose wet membranes with a diameter of 1.5 cm, place the beaker in a water bath magnetic stirrer, and the reaction conditions are 5 ° C and 20 rpm;

[0056] (2) Add 4.62mL of prepared N,N-dimethyl-N-[3-(trimethoxysilyl)propyl]octadecylammonium chloride solution to the above system with a concentration of 6.2×10 -2 mmol / L;

[0057] (3) Add glacial acetic acid dropwise to adjust the pH to 4.0, and continue stirring for 48 hours at 5°C and 20 rpm;

[0058] (4) Collecting the reacted bacterial cellulose membrane, washing with deionized water repeatedly to obtain the grafted bacterial cellulose membrane.

[0059] Characterization tests and performance experiments were carried out on the bacterial cellulose prepared by the above modification, including:

[0060] ①Compar...

Embodiment 3

[0071] The amino-functionalized bacterial cellulose material was prepared according to the following steps:

[0072] (1) Add 10 mL of deionized water to a 25 mL beaker, then add 10 pieces of purified bacterial cellulose wet membranes with a diameter of 1.5 cm, place the beaker in a water bath magnetic stirrer, and the reaction conditions are 60 ° C and 20 rpm;

[0073] (2) Add 0.23mL of prepared N,N-dimethyl-N-[3-(trimethoxysilyl)propyl]octadecylammonium chloride solution to the above system with a concentration of 3.1×10 -3 mmol / L;

[0074] (3) Add glacial acetic acid dropwise to adjust the pH to 4.0, and continue stirring at 60°C and 20 rpm for 48 hours;

[0075] (4) Collecting the reacted bacterial cellulose membrane, washing with deionized water repeatedly to obtain the grafted bacterial cellulose membrane.

[0076] Characterization tests and performance experiments were carried out on the bacterial cellulose prepared by the above modification, including:

[0077] ①Comp...

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Abstract

The invention relates to a preparation method of an antimicrobial bacterial cellulose material. The method comprises the following steps: adding an organosilicon quaternary ammonium salt into a solvent to obtain an organosilicon quaternary ammonium salt solution; and adding the organosilicon quaternary ammonium salt solution into a bacterial cellulose hydrogel, regulating the pH value to 2.0-7.0, carrying out stirring or oscillating reaction at 5-95 DEG C for 24-72 hours, and cleaning. The method is simple to operate and mild in conditions; and the grafting reaction occurs on the fiber surface, so that the grafting ratio is high. The graft-modified bacterial cellulose material has the advantages of bacterial resistance, protein adsorptivity, cell adhesion promotion characteristic, and specific pH responsivity and temperature responsivity. The antimicrobial bacterial cellulose material has excellent resistance to Staphylococcus aureus and other bacteria, is beneficial to adhesion and growth of the cells in the material, and enhances the application potential of the bacterial cellulose material in the fields of antimicrobial wound dressings, intelligent responsive drug-slow-release carriers, tissue engineering stent biomedicine and the like.

Description

technical field [0001] The invention belongs to the field of biological material preparation, in particular to a preparation method of an antibacterial bacterial cellulose material. Background technique [0002] Bacterial cellulose is nanocellulose directly synthesized and secreted by certain bacteria. Bacterial cellulose has high water content, considerable mechanical properties, and unique nanofiber network structure, making it considered as a biomedical material with broad application prospects, which can be used for tissue engineering scaffolds, artificial blood vessels, wounds Dressings, artificial cartilage joints and many other applications. However, bacterial cellulose itself has no antibacterial and antibacterial ability, which limits its application in the field of biomedicine. [0003] In the existing patents and research reports, the antibacterial and antibacterial functions of bacterial cellulose medical materials are mainly added to bacterial cellulose by add...

Claims

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Application Information

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IPC IPC(8): C08J7/12A61L15/28A61L15/20A61L15/44A61L27/20A61L27/54A61K47/38C08L1/02
CPCA61K47/38A61L15/20A61L15/28A61L15/44A61L27/20A61L27/54A61L2300/208A61L2300/232A61L2300/404C08J7/12C08J2301/02C08L1/02
Inventor 洪枫张青松张鹏袁海彬陈琳
Owner DONGHUA UNIV
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