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Kit for myocardial function assay and preparation method thereof

A technology of functional testing and kits, applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of low initial sensitivity and high specificity, and achieve the effects of high detection sensitivity, simple operation, and easy control

Active Publication Date: 2016-08-10
BEIJING MOKOBIO LIFE SCI CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

cTnI shows lower initial sensitivity and higher specificity than cTnT

Method used

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  • Kit for myocardial function assay and preparation method thereof
  • Kit for myocardial function assay and preparation method thereof
  • Kit for myocardial function assay and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] The reagent kit for detecting the function of myocardial markers in this embodiment includes a plastic plate, a glass cellulose membrane set on the plastic plate, a nitrocellulose membrane set on the glass cellulose membrane, and one end of the nitrocellulose membrane is provided with a The sample pad is provided with an absorbent pad at the corresponding other end, and the glass cellulose membrane is coated with quantum dot-labeled troponin antibody, quantum dot-labeled myoglobin antibody and quantum dot-labeled antibody in a mass ratio of 1:1:1. A mixture of dot-labeled creatine kinase antibodies; the nitrocellulose membrane is provided with a detection line 1, a detection line 2, a detection line 3 and a control line, and the detection line 1 is coated with a troponin secondary antibody, so The detection line 2 is coated with myoglobin secondary antibody, the detection line 3 is coated with creatine kinase secondary antibody, and the control line is coated with goat a...

Embodiment 2

[0066] The kit for the function detection of myocardial markers in this example differs from the example in that the quantum dots used are cadmium sulfide quantum dots, and the modified cyclodextrin used is aminated dry cyclodextrin to prepare troponin During the process of antibody, quantum dot-labeled myoglobin antibody and quantum dot-labeled creatine kinase antibody, the mixing time for adding antibody or antigen to the activated quantum dot-modified cyclodextrin-latex suspension is 2 Hour, other with embodiment 1.

Embodiment 3

[0068] The kit for detecting the function of myocardial markers in this example is different from the example in that in the process of preparing troponin antibody, quantum dot-labeled myoglobin antibody and quantum dot-labeled creatine kinase antibody, the activated The mixing time for adding the antibody or antigen to the quantum dot-modified cyclodextrin-latex suspension is 4 hours, and the others are the same as in Example 1.

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Abstract

The invention relates to the technical field of immunochromatographic assay, and particularly discloses a kit for myocardial function assay and a preparation method thereof. The kit comprises a plastic plate, a glass cellulose membrane, a nitrocellulose membrane, a sample pad and a water absorbing pad, and the glass cellulose membrane is coated with a mixture of a troponin antibody, a myoglobin antibody and a creatine jubase antibody which are labeled with quantum dots; the nitrocellulose membrane is provided with an assay line 1, an assay line 2, an assay line 3 and a control line, the assay line 1 is coated with a troponin secondary antibody, the assay line 2 is coated with a myoglobin secondary antibody, the assay line 3 is coated with a creatine jubase secondary antibody, and the control line is coated with goat anti-mouse IgG. According to the kit, a double antibody and antigen sandwich principle is combined with the fluorescence characteristic of the quantum dots, and rapid and sensitive combined assay to troponin, myoglobin and creatine jubase is achieved by means of the fluorescent immunochromatographic method.

Description

technical field [0001] The invention relates to the technical field of immunochromatography detection, in particular to a kit for myocardial function detection and a preparation method thereof. Background technique [0002] Myoglobin (Mb) is an oxygen-binding heme protein that is mainly distributed in cardiac and skeletal muscle tissues. In acute myocardial injury, Mb is first released into the blood. About 2-3 hours after the onset of symptoms, Mb in the blood can exceed the normal upper limit, reach the peak at 9-12 hours, and return to normal after 24-36 hours. Serial sampling is recommended for patients with suspected ACS because of the delay between the onset of symptoms and the release of protein markers into the blood. The clinical utility of early diagnosis and monitoring of ACS has been extensively reported in the literature. Detecting the concentration of myoglobin can be used for the early diagnosis of some diseases, such as (1) elevated blood: hypothyroidism, h...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/6887
Inventor 金鑫曾滨赵正道
Owner BEIJING MOKOBIO LIFE SCI CO LTD
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