Doped meat paste qualitative and quantitative detection method based on two-dimensional correlation infrared spectroscopy

Abstract

The invention discloses a doped meat paste qualitative and quantitative detection method based on two-dimensional correlation infrared spectroscopy. The method comprises the following steps: preparing multiple pure meat paste samples and a mixed meat paste sample of multiple doping-level meats, and acquiring mid-infrared spectra of all the samples; calculating dynamic spectrum of the mid-infrared spectra and synchronous and asynchronous two-dimensional correlation spectrum; calculating Euclidean distance between two-dimensional correlation spectra of all the pure meat paste samples to be labeled as intra-group distance; calculating Euclidean distance between two-dimensional correlation spectra of the pure meat paste samples with known doping level to be labeled as inter-group distance; detecting significance of difference between the intra-group distance and the inter-group distance, and keeping sample data with significant difference; selecting a sample to be measured, and calculating Euclidean distance between two-dimensional correlation spectra of the sample to be measured and the pure meat paste samples to be labeled as unknown distance; and observing significance level of difference between the unknown distance and the intra-group distance and all inter-group distance and judging whether the sample to be measured is doping and its doping level.

Description

technical field [0001] The invention relates to the qualitative and quantitative detection of non-trace doping substances, in particular to a qualitative and quantitative detection method for doped minced meat based on two-dimensional correlation infrared spectroscopy. Background technique [0002] The adulteration of meat and minced meat products is serious. At present, more detection methods are used, including protein and other electron focusing methods, high performance liquid chromatography, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, urea and other electrofocus methods, etc. , the application range of the above protein-related methods is relatively narrow, and it is not suitable for all kinds of meat samples, especially meat samples. Although polymerase chain reaction and DNA sequencing detection are relatively accurate, the detection reagents are expensive, the inspection cost is high, the inspection time is long, and the professional quality of the ope...

AI Technical Summary

Problems solved by technology

[0006] The object of the present invention is to address the above-mentioned problems and deficiencies, to provide a qualitative and quantitative detection method based on two-dimensional correlation infrared spectroscopy for adulterated minced meat, adopting an attenuated total reflection sampling mode to collect a series of mid-infrared spectra of samples under temperature disturbance; further Obtain two-dimensional correlation synchronous spectrum or asynchronous spectrum, and then quantify the difference between two-dimensional correlation synchronous spectrum or asynchronous spectrum of different samples, so as to achieve the purpose of qualitative and quantitative identification of samples; that is, solve the problem of high detection cost of traditional wet chemical methods, Unfriendly environment, difficult to quantify and other shortcomings, but also effectively make up for the defects of model failure of near-infrared spectroscopy modeling analysis method

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