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Grifola frondosa liquid strain culture solution and cultivation method

A technology of liquid strains and cultivation methods, applied in the directions of microorganism-based methods, botanical equipment and methods, biochemical equipment and methods, etc., can solve the problems affecting the yield of Grifola frondosa, susceptible to yellow rot virus, and rot of Grifola frondosa and other problems to achieve the effect of shortening the age and growth cycle of bacteria, shortening the cycle of bacteria, and promoting absorption

Active Publication Date: 2016-08-10
东莞市华蕈生物科技有限公司
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AI Technical Summary

Problems solved by technology

[0006] However, the liquid strain of Grifola frondosa that is cultivated by the above-mentioned liquid medium is easily infected with viruses during the cultivation process, especially easily infected with yellow rot virus, which causes large-scale rot and yellowing of Grifola frondosa, which seriously affects the quality of Grifola frondosa. output

Method used

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  • Grifola frondosa liquid strain culture solution and cultivation method
  • Grifola frondosa liquid strain culture solution and cultivation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The nutrient solution of described Grifola frondosa liquid strain comprises following components and parts by weight thereof:

[0039] 72 parts of glucose, 12 parts of soybean powder, 2 parts of potassium dihydrogen phosphate, 2 parts of magnesium sulfate, 0.05 parts of defoamer, 2 parts of growth promoter and 600 parts of water; Composition in a weight ratio of 4:3.

[0040] The cultivation method of described Grifola frondosa liquid strain:

[0041] Cultivation of strains in S1 slant test tubes: put the above-mentioned Grifola frondosa liquid strain culture solution in a test tube, then place the test tube at 123°C for 40 minutes to sterilize, after cooling, dry it in an incubator at 36°C, and put it in 2 days later. The pure strain of Grifola frondosa was inoculated on the solid medium in the inclined test tube, and cultivated for 10 days at a constant temperature of 22°C to obtain the inclined test tube strain;

[0042] Cultivation of S2 triangular flask strains: ...

Embodiment 2

[0047] The nutrient solution of described Grifola frondosa liquid strain comprises following components and parts by weight thereof:

[0048] 78 parts of glucose, 18 parts of soybean powder, 2 parts of potassium dihydrogen phosphate, 2 parts of magnesium sulfate, 0.08 parts of defoamer, 4 parts of growth promoter and 650 parts of water; Composition at a weight ratio of 5:2.

[0049] The breeding method of the Grifola frondosa liquid strain is similar to Example 1.

Embodiment 3

[0051] The nutrient solution of described Grifola frondosa liquid strain comprises following components and parts by weight thereof:

[0052] 80 parts of glucose, 20 parts of soybean powder, 4 parts of potassium dihydrogen phosphate, 5 parts of magnesium sulfate, 0.1 part of defoamer, 6 parts of growth promoter and 700 parts of water; Composition at a weight ratio of 6:1.

[0053] The breeding method of the Grifola frondosa liquid strain is similar to Example 1.

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Abstract

The invention belongs to the technical field of edible fungus cultivation and relates to a grifola frondosa liquid strain culture solution and a cultivation method. The grifola frondosa liquid strain culture solution mainly comprises glucose, soya bean powder, potassium dihydrogen phosphate, magnesium sulfate, an antifoaming agent, a growth substance and water. The grifola frondosa liquid strain culture solution accelerates a strain growth speed, improves strain activity and vitality and shortens a grifola frondosa fungus age and a growth period. The grifola frondosa liquid strain develops well, has good antibiosis and antiviral capability, has even fungus ages, grows uniformly, comprehensively improves grifola frondosa liquid strain quality and yield and reduces a production cost.

Description

technical field [0001] The invention belongs to the technical field of edible fungus cultivation, and in particular relates to a culture solution and a cultivation method of liquid strains of Grifola frondosa. Background technique [0002] Grifda frondosa (Grifda frondosa) belongs to the Basidiomycotina subphylum Phylomycetes, and the genus Polyporus of the order Aphyllomycetes. The fruiting body of Grifola frondosa is rich in essential amino acids and vitamins, and has high nutritional value, and its meat is soft, crisp and delicious when young, and is deeply loved by people. The fruiting body of Grifola frondosa also contains β-glucan, which has the functions of preventing diabetes, anti-tumor and anti-AIDS virus, and has high medicinal value. [0003] Because the quantity of wild Grifola frondosa in nature is extremely small, and presents the trend of decline year by year, its quantity can not satisfy the demand of market far away. At present, the artificial cultivation...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01G1/04C05G3/00C12R1/645
Inventor 郑列宜
Owner 东莞市华蕈生物科技有限公司
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