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an oxidase reagent

A technology of oxidase and reagents, applied in biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of unreachable reaction, fast storage time, etc., and achieve easy observation, long storage time, and fast color reaction time Effect

Active Publication Date: 2019-07-02
SHANDONG XINKE BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] But the above prior art can not reach the effect of fast response and long storage time

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0034] An oxidase reagent consisting of the following concentration percentage components:

[0035]

[0036] The oxidase reagent of this embodiment can be used to detect whether cytochrome oxidase exists in bacteria, and the color development time is fast, and the oxidase reagent has a long storage time, which can be extended from 7-14 days to one year, and will not It affects its detection effect, and the positive result is stored for a long time, and there will be no error in the detection result due to missing the result interpretation time.

[0037] The oxidase reagent in this embodiment is prepared by the following method:

[0038] The first step: preparation work, cut the paper into a suitable size;

[0039] Step 2: Weigh and dissolve according to the test formula. This solution is an oxidase reagent;

[0040] Step 3: This oxidase reagent needs to be soaked in the dark.

[0041] The oxidase reagent of this embodiment detects whether cytochrome oxidase exists in bac...

Embodiment 2

[0051] An oxidase reagent, which is basically the same as in Example 1, the difference is that the oxidase reagent in this embodiment consists of the following components:

[0052]

Embodiment 3

[0054] An oxidase reagent, which is basically the same as in Example 1, the difference is that the oxidase reagent in this embodiment consists of the following components:

[0055]

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Abstract

The invention relates to an oxidase reagent. The oxidase reagent comprises the following components of 0.5% to 2% of N,N-Dimethyl-p-phenylenediamine dihydrochloride, 0.15% to 0.35% of thiourea and 0.01% to 0.03% of vitamin C. The oxidase reagent has the advantages that the oxidase reagent is suitable for detecting whether cytochrome oxidase is contained in bacteria or not, the color rendering speed is high, and the judging time of positive result is 30s; the storage time is long, the influence to detection effect is avoided, the storage time of positive result is long, and the mistake of detection result due to missing of result judging time is avoided; the oxidase reagent is suitable for verifying strains of Neisseria, and the bacteria of Neisseria are positive; the oxidase reagent is also used for distinguishing and identifying the bacteria of Pseudomonas and Enterobacteriaceae, the bacteria of Pseudomonas are negative, and the bacteria of Enterobacteriaceae are positive; the bacteria of Moraxella and Alkaligenes are positive.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to the field of detection of cytochrome oxidase, in particular, the invention relates to an oxidase reagent. Background technique [0002] Bacterial oxidase (cytochrome oxidase) is the final respiratory enzyme of the cytochrome respiratory enzyme system, which provides energy for the life activities of bacteria. [0003] The bacterial oxidase test is one of the key tests for clinical bacteriological identification. In the prior art, the principle of the oxidase test is that the oxidase does not directly react with the oxidase reagent, but first oxidizes cytochrome C, and then the oxidase Cytochrome C oxidizes tetramethyl-p-phenylenediamine hydrochloride or dimethyl-p-phenylenediamine hydrochloride to produce a blue or red color reaction. , the bacterial oxidase test is used to detect the presence of oxidase in bacteria. [0004] However, the reagents prepared by simply utilizing tetra...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/26
CPCC12Q1/26C12Q2326/14
Inventor 庞超张会翠陶艳霞陈晓霞卢春燕
Owner SHANDONG XINKE BIOLOGICAL TECH
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