CRK4 protein and application of coded gene thereof in regulating and controlling growth of plant stems and leaves

A technique for encoding genes and plant stems and leaves, which is applied to the application field of CRK4 protein and its encoding genes in regulating the growth of plant stems and leaves. The effect of applying the foreground

Inactive Publication Date: 2016-07-27
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CRKs genes are mainly distributed on chromosome IV, and chromosome IV contains a gene cluster composed of 19 CRKs genes connected end to end, which also makes it extremely difficult to obtain crks multiple mutants

Method used

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  • CRK4 protein and application of coded gene thereof in regulating and controlling growth of plant stems and leaves
  • CRK4 protein and application of coded gene thereof in regulating and controlling growth of plant stems and leaves
  • CRK4 protein and application of coded gene thereof in regulating and controlling growth of plant stems and leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Embodiment 1, the acquisition and identification of CRK4 transgenic plants

[0051] The CRK4 gene involved in this example is derived from Arabidopsis thaliana (Arabidopsisthaliana), and its sequence in the Arabidopsis genome is shown in sequence 1 in the sequence table, and sequence 1 consists of 2641 nucleotides, which is the CRK4 The sequence of the gene in the Arabidopsis genome, in which positions 845-934, 1070-1151, 1274-1446, 1658-1747, 1986-2061 and 2219-2317 are all intron sequences; sequence 2 consists of 2031 nucleotides Composition, which is the cDNA sequence of the CRK4 gene, wherein the 1-2031st position is the coding sequence (ORF); both sequence 1 and sequence 2 encode the protein shown in sequence 3 in the sequence list, and sequence 3 consists of 676 amino acid residues .

[0052] 1. Construction of recombinant expression vector pCAMBIA-1300-221-CRK4

[0053] The total RNA of Arabidopsis wild-type (Col ecotype) was extracted, and cDNA was obtained af...

Embodiment 2

[0090] Embodiment 2, CRK4 transgenic plant seedling growth test analysis

[0091] ABA is an important signaling molecule for plants to resist external adversity stress, and has a wide range of physiological effects. ABA can promote the process of seed maturation and dormancy and the accumulation of storage proteins during seed maturation, inhibit seed germination and seedling growth, inhibit main root development, promote lateral root development, and promote leaf senescence and abscission. Therefore, in the presence of exogenous ABA, the root length and stem and leaf growth of plants are significantly inhibited. According to the sensitivity of wild-type and CRK4 transgenic plants to ABA, it can be detected whether CRK4 is involved in the process of ABA-inhibited stem and leaf growth.

[0092] The wild-type Arabidopsis (Col-0 ecotype), the single-copy inserted T obtained in Example 1 3 Seeds of homozygous C4OE-1 and C4OE-2 were sown directly on MS plates without ABA and conta...

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Abstract

The invention discloses CRK4 protein and application of a coded gene thereof in regulating and controlling the growth of plant stems and leaves.The application provided herein specifically includes: the application of a protein (CRK4 protein) formed by amino acid sequences shown by sequence 3 in a sequence table, in the following items a1) or a2): a1), inhibiting excessive growth of the plant stems and leaves; a2), breeding a plant variety with the stem and leaf excessive growth inhibited.A CRK4 gene overexpressed plant is more sensitive to ABA-inhibited excessive growth of stems and leaves, as compared to wild control plants.The inhibition on the excessive growth of stems and leaves by regulating expression level of CRK4 is of great significance, and transgenic crops with high CRK4 gene expression and stems and leaves inhibited are acquired by means of genetic engineering.The purpose of sustainable development of agriculture is satisfied, it is possible to save nutrients and reduce the spraying of chemical fertilizers and pesticides, cultivation of green pollution-free new varieties is made possible, and the invention has a promising application prospect.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to the application of a CRK4 protein and its coding gene in regulating the growth of plant stems and leaves. Background technique [0002] When planting crops, fruit trees or flower gardening plants, stems and leaves often grow excessively, resulting in a large consumption of nutrients, a reduction in the number of flower organs, a reduction in yield, and normal results cannot be guaranteed during the filling period. There are many factors that cause the stems and leaves of plants to grow excessively, including the external natural environment, such as short sunshine, weak light and excessive rainfall, etc., as well as the factors of crops or fruit tree varieties themselves. Therefore, it is of great practical significance to suppress the excessive growth of stems and leaves by improving crops, fruit trees and flower varieties. With the deepening of research on molecular biology and plan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/12C12N15/54C12N15/82A01H5/00
CPCC12N9/12C12N15/8225C12N15/8226
Inventor 张大鹏路凯王小芳
Owner TSINGHUA UNIV
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