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A kind of forensic medicine diatom test method

An inspection method and forensic technology, applied in the field of algae inspection, can solve the problems that cannot be widely used in grass-roots forensic laboratories, can not significantly improve the recovery rate of diatoms, increase the centrifugal speed, etc., and achieve easy promotion, high cost performance, and improved recovery rate Effect

Inactive Publication Date: 2018-05-18
山东政法学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to the research report of Hu Sunlin et al., one centrifugation results in about 10% diatom loss, and increasing the centrifugation speed cannot significantly improve the recovery rate of diatoms, thus resulting in a low recovery rate of diatoms in organ tissues.
[0005] Diatoms can also be enriched by vacuum filtration, but the filter membrane itself is opaque and cannot be directly observed under an optical microscope. Therefore, Hu Sunlin et al. tried to use vacuum filtration-automated scanning electron microscopy to observe diatoms
Although the electron microscope has high resolution and clear observation, due to its complex operation, high cost and high maintenance cost, the electron microscope can only be used in some large and medium-sized laboratories and scientific research institutions, and cannot be widely used in grassroots forensic science laboratory

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] S1. Take 0.15 parts of sulfamethazine, 12 parts of acrylic acid, 25 parts of acrylamide, 0.8 parts of N'N-methylenebisacrylamide, 0.8 parts of ammonium persulfate and 35 parts of deionized water and mix them fully in a beaker. After deoxygenation by nitrogen, it was filled into the splint space formed by two glass slides, and placed in an oven at 60°C for polymerization reaction for 3 hours to obtain a film-like imprinted hydrogel;

[0020] S2. Soak the imprinted hydrogel obtained in step S1 in 10% acetic acid solution, and ultrasonically vibrate for 5 minutes to remove the template molecule sulfamethazine;

[0021] S3. Soak the imprinted hydrogel obtained in step S3 in the sample to be tested for 5 minutes, take it out, and place it under an optical microscope for observation; use manual identification or computer automatic identification to identify the diatoms on the filter membrane or the pictures taken Inspection, classification and statistical processing; wherein,...

Embodiment 2

[0023] S1. Take 0.19 parts of sulfamethazine, 16 parts of acrylic acid, 35 parts of acrylamide, 1 part of N'N-methylenebisacrylamide, 1 part of ammonium persulfate and 45 parts of deionized water in a beaker and mix them thoroughly. After deoxygenation with nitrogen, it was filled into the splint space formed by two glass slides, and placed in an oven at 60°C for polymerization reaction for 3 hours to obtain a film-like imprinted hydrogel;

[0024] S2. Soak the imprinted hydrogel obtained in step S1 in 10% acetic acid solution, and ultrasonically vibrate for 510 minutes to remove the template molecule sulfamethazine;

[0025] S3. Soak the imprinted hydrogel obtained in step S3 in the sample to be tested for 10 minutes, take it out, and place it under an optical microscope for observation; use manual identification or computer automatic identification to identify the diatoms on the filter membrane or the pictures taken Inspection, classification and statistical processing; wher...

Embodiment 3

[0027] S1. Take 0.17 parts of sulfamethazine, 14 parts of acrylic acid, 30 parts of acrylamide, 0.9 parts of N'N-methylenebisacrylamide, 0.9 parts of ammonium persulfate and 40 parts of deionized water and mix them fully in a beaker. After deoxygenation by nitrogen, it was filled into the splint space formed by two glass slides, and placed in an oven at 60°C for polymerization reaction for 3 hours to obtain a film-like imprinted hydrogel;

[0028] S2. Soak the imprinted hydrogel obtained in step S1 in 10% acetic acid solution, and ultrasonically vibrate for 7.5 minutes to remove the template molecule sulfamethazine;

[0029] S3. Soak the imprinted hydrogel obtained in step S3 in the sample to be tested for 7.5 minutes, take it out, and observe it under an optical microscope; use manual identification or computer automatic identification to detect the diatoms on the filter membrane or the pictures taken Inspection, classification and statistical processing are carried out; wher...

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Abstract

The invention discloses a forensic medicine diatom test method.The forensic medicine diatom test method comprises the following steps that sulfamethazine, acrylic acid, acrylamide, N'N-methylene bisacrylamide, ammonium persulfate and deionized water are taken according to a proportion and fully mixed in a beaker; after oxygen is removed with nitrogen, the mixture is poured into a sandwich space formed by two glass slides and then put into an oven at the temperature of 60 DEG C for a polymerization reaction for 3 h, and film-state imprinting hydrogel is prepared; the obtained imprinting hydrogel is soaked in a 10% acetic acid solution, template molecules sulfadimidine in the imprinting hydrogel are removed through ultrasonic oscillation, then the imprinting hydrogel is soaked in a sample to be tested for 5-10 min and then taken out, and then the imprinting hydrogel is put under an optical microscope for observation; diatom on the filter film or a shot picture is checked, classified and counted in a manual recognition mode or an automatic computer recognition mode.The forensic medicine diatom test method is high in yield, efficiency and performance cost ratio, easy to popularize, accurate in qualitative and quantitative analysis and low in labor intensity.

Description

technical field [0001] The invention relates to the field of algae inspection, in particular to a forensic diatom inspection method. Background technique [0002] Diatom is a kind of single-celled algae. There are more than 16,000 kinds of diatoms in the world. The body length is generally between 1 μm and 200 μm. A transparent shell with shell rings fit together to form a siliceous cell wall. The living environment of diatoms is very wide, and diatoms can be found in almost all waters in nature. [0003] Dead bodies in water are a common type of forensic autopsy. Since the 1940s, many forensic scientists have confirmed on the bodies of the drowned that diatoms can enter multiple organs from the circulatory system. The diatom cell wall is not easy to be destroyed because of its strong resistance, and those with high silicon content will not be destroyed by boiling with concentrated sulfuric acid or concentrated nitric acid or even burning at high temperature. Therefore, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/84G01N1/28
CPCG01N1/28G01N21/84
Inventor 赵峰
Owner 山东政法学院
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