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Antibacterial peptide CSTC24 and application thereof

A technology of antimicrobial peptides and bacteria, applied in the fields of antimicrobial peptides and their applications, can solve problems affecting food safety and the healthy development of the aquaculture industry, pollution, excessive drug residues in the aquaculture environment, etc.

Active Publication Date: 2016-07-20
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Long-term use of antibiotics not only causes bacteria and other pathogens to develop drug resistance, but also causes excessive drug residues in animals and serious pollution of the breeding environment, which has seriously affected my country's food safety and the healthy development of the breeding industry

Method used

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  • Antibacterial peptide CSTC24 and application thereof
  • Antibacterial peptide CSTC24 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] The antimicrobial peptide CSTC24 of the present invention has the amino acid sequence in SEQ ID No. 1 of the sequence listing.

[0013] The sequence SEQIDNo.1 is: RRHCIKKCMKSRKHNERMIRIRRRK

[0014] sequence

[0015] (a) Sequence features:

[0016] Length: 24

[0017] Type: amino acid sequence

[0018] Chain type: single chain

[0019] Topology: Linear

[0020] (b) Molecule type: protein

[0021] (c) Assumption: No

[0022] (d) Antisense: no

[0023] (e) Original source: Synthetic

[0024] The antimicrobial peptide CSTC24 synthesized in this example follows the Fmoc solid-phase peptide synthesis method to finally obtain the antimicrobial peptide CSTC24 with a purity greater than 90%. During synthesis, a Fmoc-amino acid derivative is covalently cross-linked to the resin, after resin swelling, deprotection, weighing, feeding, washing after reaction, detection, washing and drying after synthesis, and then connecting the next amino acid until After connecting the l...

Embodiment 2

[0026] Application of Antimicrobial Peptide CSTC24

[0027] 1) Preparation of Vibrio vulnificus, Micrococcus luteus and Staphylococcus aureus. Culture Vibrio vulnificus, Micrococcus luteus and Staphylococcus aureus in LB medium to OD 600 was 0.80, then centrifuged at room temperature (5000g) for 10min, collected the cells, and suspended them in LB to a final concentration of 2×10 6 CFU / ml.

[0028] The PBS composition is by weight percentage: 0.8% NaCl, 0.02% KCl, 0.358%, Na 2 HPO 4 .12H 2 O, 0.024% NaH 2 PO 4 .

[0029] 2) Bactericidal activity of the antimicrobial peptide CSTC24. Add 50 μl of the bacteria solution from the above step 1) into a 96-well cell culture plate, mix it with CSTC24 or PBS diluted in multiples, and incubate at 28° C. for 24 hours. The results showed that the minimum inhibitory concentrations of CSTC24 against Vibrio vulnificus, Micrococcus luteus and Staphylococcus aureus were: 300 μM, 5 μM and 40 μM; the minimum bactericidal concentrations w...

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Abstract

The invention relates to an antibacterial peptide CSTC24 and application thereof, in particular to an antibacterial peptide.The antibacterial peptide is characterized in that the sequence of the antibacterial peptide is shown in SEQ ID No.1.The antibacterial peptide can effectively kill three fish pathogenic bacteria of vibrio vulnificus, micrococcus luteus and staphylococcus aureus, and infectious spleen and kidney necrosis viruses.The antibacterial peptide is excellent in effect through experimental verification.

Description

technical field [0001] The invention relates to the field of molecular microbiology, in particular to an antibacterial peptide and its application. Background technique [0002] In recent years, with the continuous expansion of aquaculture scale and the continuous deterioration of the aquaculture environment, various diseases have also occurred frequently. At present, the control of aquaculture diseases is still dominated by traditional antibiotics. Long-term use of antibiotics not only makes bacteria and other pathogens develop drug resistance, but also causes excessive drug residues in animals and serious pollution of the breeding environment, which has seriously affected my country's food safety and the healthy development of the breeding industry. Therefore, it has become an urgent need for the healthy development of the aquaculture industry to find antibacterial substances that can replace traditional antibiotics and achieve the goal of no pollution to the environment ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/00A61K38/16A61P31/04A61P31/12
CPCA61K38/00C07K14/00
Inventor 张敏贺淑文郝磊娜程顺峰
Owner QINGDAO AGRI UNIV
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